We describe experimental vignette methodology (EVM) as a way to address the dilemma of conducting experimental research that results in high levels of confidence regarding internal validity but is challenged by threats to external validity versus conducting nonexperimental research that usually maximizes external validity but whose conclusions are ambiguous regarding causal relationships. EVM studies consist of presenting participants with carefully constructed and realistic scenarios to assess dependent variables including intentions, attitudes, and behaviors, thereby enhancing experimental realism and also allowing researchers to manipulate and control independent variables. We describe two major types of EVM aimed at assessing explicit (i.e., paper people studies) and implicit (i.e., policy capturing and conjoint analysis) processes and outcomes. We offer best practice recommendations regarding the design and implementation of EVM studies based on a multidisciplinary literature review, discuss substant...

Best Practice Recommendations for Designing and Implementing Experimental Vignette Methodology Studies

With much advancement in the field of nanotechnology, bioengineering, and synthetic biology over the past decade, microscales and nanoscales devices are becoming a reality. Yet the problem of engineering a reliable communication system between tiny devices is still an open problem. At the same time, despite the prevalence of radio communication, there are still areas where traditional electromagnetic waves find it difficult or expensive to reach. Points of interest in industry, cities, and medical applications often lie in embedded and entrenched areas, accessible only by ventricles at scales too small for conventional radio waves and microwaves, or they are located in such a way that directional high frequency systems are ineffective. Inspired by nature, one solution to these problems is molecular communication (MC), where chemical signals are used to transfer information. Although biologists have studied MC for decades, it has only been researched for roughly 10 year from a communication engineering lens. Significant number of papers have been published to date, but owing to the need for interdisciplinary work, much of the results are preliminary. In this survey, the recent advancements in the field of MC engineering are highlighted. First, the biological, chemical, and physical processes used by an MC system are discussed. This includes different components of the MC transmitter and receiver, as well as the propagation and transport mechanisms. Then, a comprehensive survey of some of the recent works on MC through a communication engineering lens is provided. The survey ends with a technology readiness analysis of MC and future research directions.

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A Comprehensive Survey of Recent Advancements in Molecular Communication

Chemotactic bacteria are guided towards the source of a nutrient by local concentration gradients. That works on the microscopic scale, but at larger scales such local cues are unreliable pointers — for example, wind or water currents may disperse odours sought by foraging animals. Using statistical techniques, Vergassola et al. have developed a general search algorithm for movement strategies based on the detection of sporadic cues and partial information. The strategy, termed 'infotaxis' as it maximizes the expected rate of information gain, could find application in the design of 'sniffer' robots. A computational model of odour plume propagation and experimental data are used to devise a general search algorithm for movement strategies in chemotaxis, based on sporadic cues and partial information. The strategy is termed 'infotaxis' as it locally maximizes the expected rate of information gain. Chemotactic bacteria rely on local concentration gradients to guide them towards the source of a nutrient1. Such local cues pointing towards the location of the source are not always available at macroscopic scales because mixing in a flowing medium breaks up regions of high concentration into random and disconnected patches. Thus, animals sensing odours in air or water detect them only intermittently as patches sweep by on the wind or currents2,3,4,5,6. A macroscopic searcher must devise a strategy of movement based on sporadic cues and partial information. Here we propose a search algorithm, which we call ‘infotaxis’, designed to work under such conditions. Any search process can be thought of as acquisition of information on source location; for infotaxis, information plays a role similar to concentration in chemotaxis. The infotaxis strategy locally maximizes the expected rate of information gain. We demonstrate its efficiency using a computational model of odour plume propagation and experimental data on mixing flows7. Infotactic trajectories feature ‘zigzagging’ and ‘casting’ paths similar to those observed in the flight of moths8. The proposed search algorithm is relevant to the design of olfactory robots9,10,11, but the general idea of infotaxis can be applied more broadly in the context of searching with sparse information.

"Infotaxis" as a strategy for searching without gradients

A comprehensive review is presented on the development and state of the art of colorimetric and fluorometric sensor arrays. Optical arrays based on chemoresponsive colorants (dyes and nanoporous pigments) probe the chemical reactivity of analytes, rather than their physical properties. This provides a high dimensionality to chemical sensing that permits high sensitivity (often down to ppb levels), impressive discrimination among very similar analytes and exquisite fingerprinting of extremely similar mixtures over a wide range of analyte types, both in the gas and liquid phases.

https://zenodo.org/record/895426/files/article.pdf

Optical sensor arrays for chemical sensing: the optoelectronic nose

A comprehensive review on the development and state of the art of colorimetric and fluorometric sensor arrays is presented Chemical sensing aims to detect subtle changes in the chemical environment by transforming relevant chemical or physical properties of molecular or ionic species (ie, analytes) into an analytically useful output Optical arrays based on chemoresponsive colorants (dyes and nanoporous pigments) probe the chemical reactivity of analytes, rather than their physical properties (eg, mass) The chemical specificity of the olfactory system does not come from specific receptors for specific analytes (eg, the traditional lock-and-key model of substrate-enzyme interactions), but rather olfaction makes use of pattern recognition of the combined response of several hundred olfactory receptors In a similar fashion, arrays of chemoresponsive colorants provide high-dimensional data from the color or fluorescence changes of the dyes in these arrays as they are exposed to analytes This provides chemical sensing with high sensitivity (often down to parts per billion levels), impressive discrimination among very similar analytes, and exquisite fingerprinting of extremely similar mixtures over a wide range of analyte types, in both the gas and liquid phases Design of both sensor arrays and instrumentation for their analysis are discussed In addition, the various chemometric and statistical analyses of high-dimensional data (including hierarchical cluster analysis (HCA), principal component analysis (PCA), linear discriminant analysis (LDA), support vector machines (SVMs), and artificial neural networks (ANNs)) are presented and critiqued in reference to their use in chemical sensing A variety of applications are also discussed, including personal dosimetry of toxic industrial chemical, detection of explosives or accelerants, quality control of foods and beverages, biosensing intracellularly, identification of bacteria and fungi, and detection of cancer and disease biomarkers

The Optoelectronic Nose: Colorimetric and Fluorometric Sensor Arrays.

A new method for localization of an odor source is proposed. A probe with four anemometric sensors and four gas sensors has been developed so that the direction of an odor source can be determined. The anemometric sensors are used for measuring the direction of the air flow carrying odor molecules, and the gas sensors are used for detecting the gas-concentration gradient. Moreover, mounting the probe on a mobile stage with the probe under the control of a personal computer makes it possible to realize an autonomous mobile sensing system. An odor source has been successfully localized using this system in a wind tunnel.

Study of autonomous mobile sensing system for localization of odor source using gas sensors and anemometric sensors

This paper describes the development of a gas sensor system to be used as a sensing node to form a dense real-time environmental monitoring network. Moreover, a new auto-calibration method is proposed to achieve the maintenance-free operation of the sensor network. The network connectivity can be used not only for data collection but also for the calibration and diagnosis of the sensors since the measured pollutant concentrations can be easily compared through the network with nearby sensors and governmental monitoring stations. Different pollutant concentrations are usually monitored at different sites. However, a case study on local NO2 distribution has shown that there exists a special condition under which pollutant concentrations become low and uniform in a certain local area. The baseline of the gas sensor response can be adjusted in this special occasion using the pollutant concentration values reported from the neighboring environmental monitoring stations. The experimental result has shown that NO2 concentration can be measured with sufficient accuracy by incorporating appropriate temperature and humidity compensation into calibration curves. Moreover, a case study on auto-calibration demonstrates its effectiveness in keeping the measurement accuracy of the sensor system in long-term operation.

Gas sensor network for air-pollution monitoring

Robots are generally equipped with at least several different modalities of sensors. Vision and range sensors are the most popular, especially in mobile robots. On the other hand, olfaction (or chemical sensing in general) had long been ignored in the robotics community because of the technical difficulties involved in realizing artificial olfaction on robotic platforms. Over the past two decades, however, various attempts are made to use chemical sensors in robotic applications. With the help of chemical sensors, mobile robots can follow chemical trails laid on the ground, track chemical plumes to find their sources, and build distribution maps of chemical substances. This paper is intended to present a brief history and the current trends of the research in this emerging field.

Chemical Sensing in Robotic Applications: A Review

A new method to remotely locate a gas/odor source using a mobile robot with gas sensors is proposed. Although several systems to search for a source have been already reported, the source location is not known until those systems finally arrive at the location. Since the proposed method enables the estimation of the distance to the source together with its direction, the source can be remotely located. Furthermore, the release rate of the gas and the range of its distribution can be obtained using the method. This feature is realized by moving the robot and fitting the gas distribution model to the gas sensor response at each location, taking the response delay into account. The soundness of the method is shown by the experiments on identification of an ethanol-gas-source location in a clean room.

Remote sensing of gas/odor source location and concentration distribution using mobile system

Many animals have the ability to search for odor sources by tracking their plumes. Some of the key features of this search behavior have been successfully transferred to robot platforms, although the capabilities of animals are still beyond the current level of sensor technologies. The examples described in this paper are (1) incorporating into a wheeled robot the upwind surges and casting used by moths in tracking pheromone plumes, (2) extracting useful information from the response patterns of a chemical sensor array patterned after the spatially distributed chemoreceptors of some animals, and (3) mimicking the fanning behavior of silkworm moths to enhance the reception of chemical signals by drawing molecules from one direction. The achievements so far and current efforts are reviewed to illustrate the steps to be taken toward future development of this technology.

Hiroshi Ishida

Papers

Study of autonomous mobile sensing system for localization of odor source using gas sensors and anemometric sensors

Gas sensor network for air-pollution monitoring

Chemical Sensing in Robotic Applications: A Review

Remote sensing of gas/odor source location and concentration distribution using mobile system

Plume-Tracking Robots: A New Application of Chemical Sensors