Hiroshi Uezato

TL;DR: A polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies, which will be a powerful tool for the rapid identification of prevalent sand fly and Leishmaniasis species as well as monitoring the infection rate in sand fly populations in endemic areas.

TL;DR: The nucleotide sequences of the Cyt b gene from 13 human-infecting Leishmania species (14 strains) from the New and Old Worlds were determined, and they showed good agreement with the classification of Lainson & Shaw (1987) except for the inclusion of L. (L.) major in the L.

TL;DR: Cyt b gene analysis is applicable to the analyzing phylogeny of the genus Leishmania and may be useful for separating non-human pathogenic species from human pathogenicspecies.

TL;DR: A method for the mass screening of sand flies for Leishmania infection was established, and 5 positive sand flies were detected, and all the positive flies were Lu.

TL;DR: Recently, genetic analyses have been performed on sand fly species and genotyping using PCR-RFLP has been applied to the sand fly taxonomy, and a molecular mass screening method has been established that enables both sandFly species and natural leishmanial infections to be identified simultaneously in hundreds of sand flies with limited effort.