The present historical moment may seem a particularly inopportune time to review Bowling Alone, Robert Putnam's latest exploration of civic decline in America. After all, the outpouring of volunteerism, solidarity, patriotism, and self-sacrifice displayed by Americans in the wake of the September 11 terrorist attacks appears to fly in the face of Putnam's central argument: that \"social capital\" -defined as \"social networks and the norms of reciprocity and trustworthiness that arise from them\" (p. 19)'has declined to dangerously low levels in America over the last three decades. However, Putnam is not fazed in the least by the recent effusion of solidarity. Quite the contrary, he sees in it the potential to \"reverse what has been a 30to 40-year steady decline in most measures of connectedness or community.\"' As an example of how the current \"war on terrorism\" could generate a durable civic renewal, Putnam points to the burst in civic practices that occurred during and after World War II, which he says \"permanently marked\" the generation that lived through it and had a \"terrific effect on American public life over the last half-century.\" 3 If Americans can follow this example and channel their current civic

Bowling alone: The collapse and revival of American community

Recording of presentation introducing narrative analysis, outlining what it is, why it can be a useful approach, how to do it and where to find out more. Presentation given at methods@manchester seminar at University of Manchester on 4 March 2010.

What is Narrative Analysis

The accumulation of unfolded proteins in the endoplasmic reticulum (ER) represents a cellular stress induced by multiple stimuli and pathological conditions. These include hypoxia, oxidative injury, high-fat diet, hypoglycaemia, protein inclusion bodies and viral infection. ER stress triggers an evolutionarily conserved series of signal-transduction events, which constitutes the unfolded protein response. These signalling events aim to ameliorate the accumulation of unfolded proteins in the ER; however, when these events are severe or protracted they can induce cell death. With the increasing recognition of an association between ER stress and human diseases, and with the improved understanding of the diverse underlying molecular mechanisms, novel targets for drug discovery and new strategies for therapeutic intervention are beginning to emerge.

Cell death and endoplasmic reticulum stress: disease relevance and therapeutic opportunities

Inorganic phosphate (Pi)-signaling pathways in plants are still largely unknown. The Arabidopsis (Arabidopsis thaliana) pho2 mutant overaccumulates Pi in leaves in Pi-replete conditions. Micrografting revealed that a pho2 root genotype is sufficient to yield leaf Pi accumulation. In pho2 mutants, Pi does not repress a set of Pi starvation-induced genes, including AtIPS1, AT4, and Pi transporters Pht1;8 and Pht1;9. Map-based cloning identified PHO2 as At2g33770, an unusual E2 conjugase gene. It was recently shown that Pi deprivation induces mature microRNA (miRNA [miR399]) and that overexpression of miR399 in Pi-replete conditions represses E2 conjugase expression and leads to high leaf Pi concentrations, thus phenocopying pho2. We show here that miR399 primary transcripts are also strongly induced by low Pi and rapidly repressed after addition of Pi. PHO2 transcripts change reciprocally to miR399 transcripts in Pi-deprived plants and in miR399 overexpressers. However, responses after Pi readdition and in β-glucuronidase reporter lines suggest that PHO2 expression is also regulated by Pi in a manner unrelated to miR399-mediated transcript cleavage. Expression of miR399 was strongly reduced in Pi-deprived Arabidopsis phr1 mutants, and a subset of Pi-responsive genes repressed in Pi-deprived phr1 mutants was up-regulated in Pi-replete pho2 mutants. This places miR399 and PHO2 in a branch of the Pi-signaling network downstream of PHR1. Finally, putative PHO2 orthologs containing five miR399-binding sites in their 5′-untranslated regions were identified in other higher plants, and Pi-dependent miR399 expression was demonstrated in rice (Oryza sativa), suggesting a conserved regulatory mechanism.

PHO2, MicroRNA399, and PHR1 Define a Phosphate-Signaling Pathway in Plants

Several of the inhibitor of apoptosis protein (IAP) family members regulate apoptosis in response to various cellular assaults. Some members are also involved in cell signalling, mitosis and targeting proteins to the ubiquitin-proteasome degradation machinery. The most intensively studied family member, X-linked IAP (XIAP), is a potent inhibitor of caspase activity; hence, it is generally assumed that direct caspase inhibition is an important conserved function of most members of the family. Biochemical and structural studies have precisely mapped the elements of XIAP required for caspase inhibition. Intriguingly, these elements are not conserved among IAPs. Here, we review current knowledge of the caspase-inhibitory potential of the human IAPs and show that XIAP is probably the only bona fide caspase inhibitor, suggesting that the other family members never gained the ability to directly inhibit caspase activity.

/pdf/human-inhibitor-of-apoptosis-proteins-why-xiap-is-the-black-qh5m0dvj3h.pdf

Human inhibitor of apoptosis proteins: why XIAP is the black sheep of the family

Apollon (also known as BRUCE or BIRC6) is a large protein containing baculoviral-IAP-repeat (BIR) and ubiquitin-conjugating enzyme (UBC) domains at the amino- and carboxy termini, respectively. Apollon inhibits apoptosis, but its molecular and physiological function remains unclear. Here we report that Apollon binds to, ubiquitinates and facilitates proteasomal degradation of SMAC and caspase-9, which both contain IAP-binding motifs. Targeted disruption of Apollon in mice caused embryonic and neonatal lethality. Notably, SMAC induced apoptosis in Apollon-deficient cells, but not in Apollon-expressing cells. Furthermore, the IAP-binding motif of SMAC was required to induce apoptosis in Apollon-deficient cells. These results suggest that Apollon has an essential function in preventing SMAC-induced apoptosis.

Apollon ubiquitinates SMAC and caspase-9, and has an essential cytoprotection function

The traditional knowledge of indigenous people is often neglected despite its significance in combating climate change. This study uncovers the potential of traditional ecological knowledge (TEK) from the perspective of indigenous communities in Sarawak, Malaysian Borneo, and explores how TEK helps them to observe and respond to local climate change. Data were collected through interviews and field work observations and analysed using thematic analysis based on the TEK framework. The results indicated that these communities have observed a significant increase in temperature, with uncertain weather and seasons. Consequently, drought and wildfires have had a substantial impact on their livelihoods. However, they have responded to this by managing their customary land and resources to ensure food and resource security, which provides a respectable example of the sustainable management of terrestrial and inland ecosystems. The social networks and institutions of indigenous communities enable collective action which strengthens the reciprocal relationships that they rely on when calamity strikes. Accordingly, the communities maintain their TEK through cultural festivals and oral traditions passed from one generation to another. TEK is a practical tool that helps indigenous communities adapt to climate risks and promotes socio-ecological resilience, which upholds social empowerment and sustainable resource management.

https://www.mdpi.com/2071-1050/12/2/676/pdf

Adaptation to climate change: Does traditional ecological knowledge hold the key?

Collective states of 110Cd, 114Cd and 116Cd excited in inelastic scattering of 55 MeV protons

Differential cross sections of the inelastic proton scattering for low lying states of 90 Zr and 92 Zr were measured. The incident proton energy was about 14.5 MeV. Inelastic groups were analyzed with a precision magnetic analyzer. Attempts of DWBA fits were made. The general trend of experimental results is in agreement with that of the work of Corolado-ORNL group.

Hitoshi Nakamura

Papers

Apollon ubiquitinates SMAC and caspase-9, and has an essential cytoprotection function

Adaptation to climate change: Does traditional ecological knowledge hold the key?

Collective states of 110Cd, 114Cd and 116Cd excited in inelastic scattering of 55 MeV protons

Inelastic Proton Scattering from 90 Zr and 92 Zr

Land use and management in Benin Republic: An evaluation of the effectiveness of Land Law 2013-01