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I-Hsuan Chen
Researcher at Regeneron
Publications - 9
Citations - 535
I-Hsuan Chen is an academic researcher from Regeneron. The author has contributed to research in topics: Glycoproteomics & Phosphoproteomics. The author has an hindex of 7, co-authored 9 publications receiving 312 citations. Previous affiliations of I-Hsuan Chen include Purdue University & Russian Academy of Sciences.
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Journal ArticleDOI
Phosphoproteins in extracellular vesicles as candidate markers for breast cancer
I-Hsuan Chen,Liang Xue,Chuan-Chih Hsu,Juan Sebastian Paez,Li Pan,Hillary Andaluz,Michael K. Wendt,Anton Iliuk,Jian-Kang Zhu,Jian-Kang Zhu,W. Andy Tao +10 more
TL;DR: A general strategy to isolate and identify phosphoproteins in extracellular vesicles from human plasma as potential markers to differentiate disease from healthy states is presented and may transform cancer screening and monitoring.
Journal ArticleDOI
Universal Plant Phosphoproteomics Workflow and Its Application to Tomato Signaling in Response to Cold Stress.
Chuan-Chih Hsu,Yingfang Zhu,Yingfang Zhu,Yingfang Zhu,Justine V. Arrington,Juan Sebastian Paez,Pengcheng Wang,Pengcheng Wang,Peipei Zhu,I-Hsuan Chen,Jian-Kang Zhu,Jian-Kang Zhu,Jian-Kang Zhu,W. Andy Tao +13 more
TL;DR: A universal sample preparation procedure that facilitates plant phosphoproteomic profiling and a streamlined approach revealed a global view of tomato cold-induced signaling mechanisms, creating the largest tomato phosphopeptides resource to date.
Journal ArticleDOI
Sequential phosphoproteomics and N-glycoproteomics of plasma-derived extracellular vesicles.
TL;DR: This protocol describes a mass spectrometry–based workflow for combined analysis of protein phosporylation and N-glycosylation of extracellular vesicles obtained from a single blood plasma sample, enabling multiple PTM analyses of the same clinical samples.
Journal ArticleDOI
Analytical Pipeline for Discovery and Verification of Glycoproteins from Plasma-Derived Extracellular Vesicles as Breast Cancer Biomarkers.
I-Hsuan Chen,Hillary Andaluz Aguilar,J. Sebastian Paez Paez,Xiaofeng Wu,Li Pan,Michael K. Wendt,Anton Iliuk,Ying Zhang,W. Andy Tao +8 more
TL;DR: This study identified 1,453 unique glycopeptides representing 556 glycoproteins in EVs and applied a novel glyco-specific reverse phase protein array to quantify a subset of the candidates, demonstrating the great potential of this integrated pipeline for biomarker discovery.
Journal ArticleDOI
Improved Host Cell Protein Analysis in Monoclonal Antibody Products through Molecular Weight Cutoff Enrichment
TL;DR: This work reports a simple and powerful strategy to identify HCPs in antibody drug substance by applying molecular weight cut-off (MWCO) filtration step followed by shotgun proteomic analysis, which doubles the number of identified H CPs that have been previously reported.