Ines Swoboda

TL;DR: The allergen microarray allows the determination and monitoring of allergic patients’ IgE reactivity profiles to large numbers of disease‐causing allergens by using single measurements and minute amounts of serum, and may change established practice in allergy diagnosis, prevention, and therapy.

TL;DR: RCyp c 1.01 reacted with IgE from all fish-allergic patients tested, induced specific and dose-dependent basophil histamine release, and contained most of the IgE epitopes present in natural allergen extracts from cod, tuna, and salmon, suggesting it may be used to identify patients suffering from IgE-mediated fish allergy.

TL;DR: High performance liquid chromatography and plasma desorption mass spectrometry of proteolytic fragments of purified natural Bet v 1 and rBet v 1a were used to confirm the primary structure of all Bet v 2 isoforms and to investigate any possible postsynthetic modifications on rBetv 1a or on the natural mixture of isoallergens obtained from birch pollen.

TL;DR: This study identified profilin as an actin-binding protein in higher plants which is present in monocot and dicot angiosperms and one of the blocking antibodies was raised against the 25 COOH-terminal amino acids indicating the importance of this region in the profilactin complex formation.

TL;DR: Immunofluorescence and confocal laser scanning microscopy showed a homogeneous distribution of profilin in the cytoplasm of in vitro cultured pollen grains and pollen tubes of tobacco whereas some growing pollen tubes were stained more intensively a their tip.