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Isabelle Léger-Silvestre
Researcher at Centre national de la recherche scientifique
Publications - 17
Citations - 1009
Isabelle Léger-Silvestre is an academic researcher from Centre national de la recherche scientifique. The author has contributed to research in topics: Nucleolus & RNA polymerase I. The author has an hindex of 15, co-authored 17 publications receiving 963 citations. Previous affiliations of Isabelle Léger-Silvestre include University of Regensburg & Paul Sabatier University.
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Journal ArticleDOI
Specific Role for Yeast Homologs of the Diamond Blackfan Anemia-associated Rps19 Protein in Ribosome Synthesis
Isabelle Léger-Silvestre,Jacqueline Marie Caffrey,Rosie Dawaliby,Diana Alehandrovna Alvarez-Arias,Nicole Gas,Salvatore Bertolone,Pierre-Emmanuel Gleizes,Steven R. Ellis +7 more
TL;DR: A pivotal role is uncovered in the assembly and maturation of the pre-40 S particles and for the first time the effect of Diamond Blackfan anemia-associated mutations on the function of Rps19 is demonstrated, strongly connecting the pathology to ribosome biogenesis.
Journal ArticleDOI
The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast
Isabelle Léger-Silvestre,Philipp Milkereit,Sébastien Ferreira-Cerca,Cosmin Saveanu,Jean-Claude Rousselle,Valérie Choesmel,Cécile Guinefoleau,Nicole Gas,Pierre-Emmanuel Gleizes +8 more
TL;DR: It is concluded that, during the final maturation steps in the nucleus, incorporation of the ribosomal protein Rps15p is specifically required to render the pre‐40S particles competent for translocation to the cytoplasm.
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The carboxy-terminal extension of yeast ribosomal protein S14 is necessary for maturation of 43S preribosomes.
Jelena Jakovljevic,Pamela Antúnez de Mayolo,Tiffany D. Miles,Theresa Mai-Ly Nguyen,Isabelle Léger-Silvestre,Nicole Gas,John L. Woolford +6 more
TL;DR: These studies are one of the first to relate the structure of eukaryotic ribosomes to their assembly pathway-the carboxy-terminal extension of rpS14 is located in the 40S subunit near the 3' end of 18S rRNA, consistent with a role for rp S14 in3' end processing of 20S pre-rRNA.
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Functional compartmentalization of the nucleus in the budding yeast Saccharomyces cerevisiae.
TL;DR: By combining cryofixation and cryosubstitution in a structural and functional analysis of the nucleus of Saccharomyces cerevisiae, morphological subcompartments in the nucleolus are identified and it is suggested that ribosomal transcripts are distributed along a nucleolar network that might include both DFC and GC.
Journal ArticleDOI
RNA polymerase I–specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle
Benjamin Albert,Isabelle Léger-Silvestre,Christophe Normand,Martin K. Ostermaier,Jorge Perez-Fernandez,Kostya I. Panov,Joost C. B. M. Zomerdijk,Patrick Schultz,Olivier Gadal +8 more
TL;DR: The Pol I subunits Rpa34 and Rpa49, required for elongation and 35S rRNA production, promote clustering of neighboring Pol I complexes to enhance the rRNA gene transcription cycle.