J.H.J. Hoeijmakers

TL;DR: Targeted inactivation of ATR in mice by disruption of the kinase domain leads to early embryonic lethality before embryonic day 8.5 (E8.5), supporting the idea that loss of both alleles of the ATR gene, even at the ES-cell level, is lethal.

TL;DR: The phenotype of the first animal mutant in the ubiquitin pathway is reported: inactivation of the hHR6B-homologous gene in mice causes male infertility, and this findings provide a parallel between yeast sporulation and mammalian spermatogenesis and strongly implicate h HR6-dependent ubiquitination in chromatin remodeling.

TL;DR: Results of a topological assay suggested that purified human Rad54 (hRad54) protein can unwind double-stranded DNA at the expense of ATP hydrolysis, which could promote the formation or stabilization of hRad51-mediated joint molecules.

TL;DR: Complementation analysis in vitro of rodent CGs is accomplished by pairwise mixing of mutant extracts, and results show that mutants from groups 2, 3, 5 and XP‐A can complement all other CGs tested, however, selective non‐complementation in vitro was observed in mutual mixtures of groups 1, 4, 11 andXP‐F, suggesting that the complementing activities involved somehow affect each other.

TL;DR: Differential postmeiotic expression of the Y chromosomal genes Ubely and Sry is shown, with highest mRNA levels in round spermatids and cytoplasmic fragments, respectively, which may have implications for the understanding of X chromosome inactivation during sperMatogenesis and paternal genome imprinting.