Showing papers in "Current Biology in 1999"

TL;DR: It is shown that Akt can regulate signaling pathways that lead to induction of the NF-κB family of transcription factors in the Jurkat T-cell line, uncovering a previously unappreciated connection between Akt and NF-kkB induction that could have implications for the control of T- cell growth and survival.

TL;DR: The data suggest that SCF(Skp2) specifically targets p27 for degradation during cell-cycle progression, and indicates that this protein complex is implicated in control of the G1-S transition in the cell cycle.

TL;DR: Several genes that are constitutively overexpressed in senescent fibroblasts are regulated during the cell cycle in early-passage cells, suggesting that senescent cells are locked in an activated state that mimics the early remodeling phase of wound repair.

TL;DR: It is concluded that β-TrCP is a component of an E3 ubiquitin ligase that is responsible for the targeted degradation of phosphorylated β-catenin.

TL;DR: It is shown that focal complexes formed in response to Rac differentiated into focal contacts upon upregulation of Rho, and that the transition from a focal complex to a focal contact is associated with a switch to Rho-kinase dependence.

TL;DR: It is shown that PCs in the developing mouse cerebellum express the gene encoding the morphogen Sonic hedgehog (Shh) and that dividing cells in the EGL express Patched and Gli1, two target genes of which expression is upregulated in response to Hedgehog signalling.

TL;DR: A model is proposed in which lamellar cytoplasm is considered a dynamically bistable system capable of existing in a non-polarized or polarized state and interconvertible by mechanical stimulus and explains how the anisotropic organization of the lamellum is maintained in the process of locomotion.

TL;DR: It is demonstrated that some but not all Wnt and Frizzled signals modulate PKC localization and stimulate PKC activity via a G-protein-dependent mechanism, and these data support the existence of multiple Wnt/Frizzled signaling pathways in vertebrates.

TL;DR: The results strongly suggest that Akt has an important role in the regulation of normal angiogenesis and raise the possibility that the enhanced activity of this kinase that occurs in carcinomas may contribute to tumor vascularization and survival.

TL;DR: PDK1 and PDK2 might be the same enzyme, the substrate specificity and activity of PDK1 being regulated through its interaction with another protein(s), and PRK2 is a probable substrate for PDK 1.

TL;DR: It is found that Hct1 was phosphorylated in vivo at multiple CDK consensus sites during cell cycle stages when activity of the cyclin-dependent kinase Cdc28 is high and APC activity is low, and phosphorylation abolished the ability of HCT1 to activate the APC in vitro.

TL;DR: The results show that a functional form of sFasL was generated by the action of the metalloproteinase matrilysin, and suggest that matRIlysin cleavage of FasL is an important mediator of epithelial cell apoptosis.

TL;DR: A family of 26 human F-box proteins, 25 of which were novel, were identified and identified by using Skp1 as a bait in a yeast two-hybrid screen and by searching DNA databases, and were named.

TL;DR: The identification of a family of 33 novel mammalian F-box proteins that may represent a new protein-protein interaction motif is reported, suggesting that the SCF system controls a correspondingly large number of regulatory pathways in vertebrates.

TL;DR: It is suggested that a significant defect in NHEJ that leads to pronounced radiosensitivity is compatible with normal human viability and does not cause any major immune dysfunction, however, the defect may confer a predisposition to leukaemia.

TL;DR: A new family of highly conserved inositol-polyphosphate kinases that contain a newly identified, unique consensus sequence are identified.

TL;DR: Evidence is presented that the role of Ran in spindle assembly is independent of nuclear transport and is probably mediated through changes in microtubule dynamics, as well as how increased concentrations of RanBP1 disturb mitosis.

TL;DR: These findings provide genetic evidence that establishes the unique role played by Mek1 in signal transduction and suggest that mek1 function is required for normal response to angiogenic signals that might promote vascularization of the labyrinthine region of the placenta.

TL;DR: It is demonstrated that Smo is the signaling component of a multicomponent Hh receptor complex and that Ptc is a ligand-regulated inhibitor of Smo.

TL;DR: The CD4-mediated 'envelope interference' described here probably explains the plurality of mechanisms developed by HIV to downregulate the cell-surface expression of its receptor.

TL;DR: It is shown that centrosome duplication requires cyclin-dependent kinase 2 (Cdk2) activity in mammalian cells, and an underlying mechanism for the coordination of centrosomes duplication with the cell cycle is suggested.

TL;DR: It is demonstrated that Gads plays an important role in T-cell signaling via its association with SLP-76 and LAT, and may promote cross-talk between the LAT and SLp-76 signaling complexes, thereby coupling membrane-proximal events to downstream signaling pathways.

TL;DR: This work demonstrates that hydrophobins not only have a function at the hyphal surface but also at the medium-air interface, which explains why fungi secrete large amounts of hydrophobin into their aqueous surroundings.

TL;DR: JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptotic induced byAnti-Fas antibody, UVC or dexamethasone, and is not required for activation-induced cell death of mature T cells.

TL;DR: It is reported that, when caspase activity was inhibited using drugs or when apoptosis was prevented genetically, interdigital cell death still occurred and this cell death was negative for the terminal-deoxynucleotidyl-mediated dUTP nick end-labelling (TUNEL) assay and there was no overall cell condensation.

TL;DR: It is reported that Brca2(Tr2014/Tr2014) MEFs frequently develop micronuclei, suggesting a potential mechanism whereby loss of BRCA2 may, within subclones, drive the loss of cell-cycle regulation genes, enabling proliferation and tumourigenesis.

TL;DR: It is reported that a zebrafish Sox17 homologue is expressed during gastrulation exclusively in the endoderm and that casanova mutants lack all sox17 expression, and it is shown that the Nodal-related molecules Cyclops and Squint and the transmembrane protein Oep are essential for normal mixer expression.

TL;DR: The results suggest that, in human cells, the DNA-damage checkpoint involves direct inactivation of Cdc25 catalyzed by Cds1 and/or Chk1, which is homologous to the CDS1/Rad53 family of checkpoint kinases in yeast.

TL;DR: The effects of caffeine on the G2/M DNA-damage checkpoint in human cells are investigated and it is reported that the radiation-induced activation of the kinase Cds1 (also known as Chk2) is inhibited by caffeine in vivo and that ATM kinase activity is directly inhibited by espresso in vitro.

TL;DR: This text introduces current evolutionary game theory--where ideas from evolutionary biology and rationalistic economics meet--emphasizing the links between static and dynamic approaches and noncooperative game theory.