J
J. Richard McIntosh
Researcher at University of Colorado Boulder
Publications - 128
Citations - 11867
J. Richard McIntosh is an academic researcher from University of Colorado Boulder. The author has contributed to research in topics: Microtubule & Spindle apparatus. The author has an hindex of 56, co-authored 128 publications receiving 11026 citations.
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Journal ArticleDOI
The molecular architecture of axonemes revealed by cryoelectron tomography.
Daniela Nicastro,Cindi L. Schwartz,Jason Pierson,Richard Gaudette,Mary E. Porter,J. Richard McIntosh +5 more
TL;DR: The three-dimensional structure of rapidly frozen axonemes from Chlamydomonas and sea urchin sperm is described using cryoelectron tomography and image processing to focus on the motor enzyme dynein, and images suggest a model for the way Dynein generates force to slide microtubules.
Journal ArticleDOI
A standardized kinesin nomenclature.
Carolyn J. Lawrence,R. Kelly Dawe,Karen R. Christie,Don W. Cleveland,Scott C. Dawson,Sharyn A. Endow,Lawrence S.B. Goldstein,Holly V. Goodson,Nobutaka Hirokawa,Jonathon Howard,Russell L. Malmberg,J. Richard McIntosh,Harukata Miki,Timothy J. Mitchison,Yasushi Okada,Anireddy S. N. Reddy,William M. Saxton,Manfred Schliwa,Jonathan M. Scholey,Ronald D. Vale,Claire E. Walczak,Linda Wordeman +21 more
TL;DR: A standardized kinesin nomenclature based on 14 family designations is set forth, which unifies all previous phylogenies and nomenClature proposals, while allowing individual sequence names to remain the same, and for expansion to occur as new sequences are discovered.
Journal ArticleDOI
Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell
Mark S. Ladinsky,David N. Mastronarde,J. Richard McIntosh,Kathryn E. Howell,L. Andrew Staehelin +4 more
TL;DR: Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at ∼7-nm resolution, placing structural constraints on models of trafficking to, through, and from the GolGI complex.
Journal ArticleDOI
Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor Nocodazole: Nocodazole accumulated mitotic cells
TL;DR: Nocodazole has very little effect on interphase metabolism, and following drug release, cells return to a normal cell cycle.
Journal ArticleDOI
A plus-end-directed motor enzyme that moves antiparallel microtubules in vitro localizes to the interzone of mitotic spindles
TL;DR: The gene that encodes this antigen contains a domain with strong sequence similarity to the motor domain of kinesin-like proteins, and the product of this gene, expressed in bacteria, can cross-bridge antiparallel microtubules in vitro, and in the presence of Mg–ATP,microtubules slide over one another in a fashion reminiscent of microtubule movements during spindle elongation.