Showing papers by "Jacques Neefjes published in 1989"
TL;DR: This study quantifies the antigen distribution in the macrophage fractions of several lymphoid organs provided by Percoll centrifugation in the rat with four new monoclonal antibodies raised against macrophages.
Abstract: A set of three monoclonal antibodies (MoAbs), ED1, ED2, and ED3, has been shown to recognize in situ different subsets of macrophages in the rat. This macrophage diversity can be correlated with differences in stage of differentiation of cells belonging to one lineage. The present study quantifies this antigen distribution in the macrophage fractions of several lymphoid organs provided by Percoll centrifugation. Four new MoAbs (ED4, ED7, ED8, and ED9) raised against macrophages are included in this study. The tissue distribution of each of the four new MoAbs is determined by immuno- and enzymehistochemistry on cryostat sections.The MoAbs recognize distinct subpopulations of macrophages. The new MoAbs ED4, ED7, ED8, and ED9 recognize granulocytes and other unrelated cell types, as well as cells of the mononuclear phagocyte system. ED7 and ED8 recognize a surface heterodimer of M, 160,000 and 95,000.
131 citations
TL;DR: It is reported that the inability to mount these specific immune responses is restored by the use of dendritic cells (DC) as antigen‐presenting cells (APC) and that removal of sialic acids, by neuraminidase, can restore specific responses to nonresponder APC as well.
Abstract: Two class I major histocompatibility (MHC) mutant mouse strains, H-2bm14 and H-2bm6, differ from the strain of origin C57BL/6 (B6, H-2b) in one and two amino acids of the H-2Db and H-2Kb molecule, respectively. The bm14 Db mutation results in specific failure of female bm14 mice to generate a cytotoxic T lymphocyte (Tc) response to the male-specific antigen H-Y. The allospecific Tc response of CD8+ B6T cells against bm6 Kb mutant spleen cells, in contrast to that against other Kb mutants, is absolutely CD4+ T helper cell dependent. Purified CD8+ T cells completely fail to respond. We now report that the inability to mount these specific immune responses is restored by the use of dendritic cells (DC) as antigen-presenting cells (APC). Comparison of MHC expression on various types of APC by cytofluorimetry and quantitative immunoprecipitation showed very high expression of class I and class II MHC molecules on DC. Strikingly, examination of class I and class II molecules by isoelectric focusing revealed qualitative differences as well. We show that the surface MHC class I molecules of DC are present in greater quantity and carry on average fewer sialic acids than the same molecules isolated from other APC types such as spleen cells, lipopolysaccharide blasts or concanavalin A blasts. That sialic acids on cell surface molecules, including MHC, may play a role in antigen presentation is suggested by our finding that removal of sialic acids, by neuraminidase, can restore specific responses to nonresponder APC as well.
85 citations
TL;DR: The HLA-A11 negative phenotype is due to a regulatory phenomenon, rather than a structural defect, as proven by the ability to rescue expression of HLA -A11 in in vitro Epstein-Barr virus (EBV)-converted sublines of EBV negative BLs.
32 citations
Journal Article•
TL;DR: The presence of these class II gene products on almost all lymphocytes in the dog is in sharp contrast to the numerous reports on their restricted expression in other mammalia such as man and mouse.
Abstract: We have analyzed the products of the canine DLA-B gene locus by biochemical and serological methods. Due to its ubiquitous expression on lymphocytes this antigen series was thought to be of class I nature. However, by two-dimensional gel electrophoresis and lysostrip experiments we could unequivocally identify the DLA-B antigens as typical class II gene products. The serological polymorphism is caused by the beta-chain, whereas the alpha-chain appears invariant. The presence of these class II gene products on almost all lymphocytes in the dog is in sharp contrast to the numerous reports on their restricted expression in other mammalia such as man and mouse.
18 citations
TL;DR: A 3H label was introduced at the C-1 position of the mannosidase I inhibitor 1-deoxymannojirimycin (dMM) by catalytic hydrogenolysis of benzyl-2,3-O-isopropylidene-5-N-benzyl-6- O-benZyl-alpha-D-mannofurano side with 3H2.
16 citations
TL;DR: The absence of sialic acid residues can explain the increased binding of anti-MHC antibodies, because neuraminidase treatment of T and Epstein-Barr virus-transformed B cells resulted in a shift in both isoelectric point and antibody binding similar to that observed after influenza virus infection.
5 citations
TL;DR: In this article, an easy accessible benzyl 2,3-O-isopropylidene-α-D-mannofuranoside (1) was converted in six steps into benzyl2,3O-Iisopricidene5-N-benzyl-5-deoxy-6-O-,O-B-I-IISOPropylIDene6-Dmannofuranide (2) in an overall yield of 38% based on 1.
Abstract: Easily accessible benzyl 2,3-O-isopropylidene-α-D-mannofuranoside (1) was converted in six steps into benzyl 2,3-O-isopropylidene-5-N-benzyl-5-deoxy-6-O-benzyl-α-D-mannofuranoside or benzyl 2,3-O-isopropylidene-5-azido-5-deoxy-6-O-benzyl-α-D-mannofuranoside. Both compounds afforded, after hydrogenolysis and acidolysis, 1-deoxymannojirimycin in an overall yield of 38% based on 1.
01 Jan 1989
TL;DR: Class I antigens deriving from classical or nonclassical major histocompatibility complex class I genes have been detected in sera of many different species but have not been analyzed biochemically in detail yet.
Abstract: Class I antigens deriving from classical or nonclassical major histocompatibility complex class I genes have been detected in sera of many different species (1). Those present in human serum — as inferred from microcytotoxicity-inhibition assays (2) — have not been analyzed biochemically in detail yet.