Jaemog Soh

TL;DR: It is confirmed that an additional factor encoded on human chromosome 21 is required for reconstitution of antiviral activity against EMCV, and that this accessory factor belongs to a family of such accessory factors responsible for different actions of IFN-γ.

TL;DR: The human SHP gene was specifically expressed in fetal liver, fetal adrenal gland, adult spleen, and adult small intestine and the activity of the mouse SHP promoter measured by transient transfection was significantly higher in the adrenal-derived Y1 cells than HeLa cells.

TL;DR: The Hu-IFN-alpha R1 subunit plays a critical role in the functional human Type I IFN receptor complex, whose components are encoded on this YAC, and as binding of ligands is retained in the cells containing the YAC with the deletion, it is clear a second subunit encoded on theYAC is responsible for ligand binding activity.

TL;DR: It is reported that a 540-kb yeast artificial chromosome encodes the necessary species-specific factor(s) and can substitute for human chromosome 21 to reconstitute the Hu-IFN-gamma-receptor-mediated induction of class I HLA antigens, however, the factor encoded on the yeast artificial chromosomes does not confer antiviral protection against encephalomyocarditis virus.

TL;DR: The results indicate that all the genes necessary to reconstitute a biologically active Type I human IFN receptor complex are located within the human DNA insert of this YAC clone.