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A simple method for displaying the hydropathic character of a protein

Overlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7 Transcripts, approximately 6500 nucleotides in size, were detectable in the tissues affected in patients with CF The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients

https://science.sciencemag.org/content/sci/245/4922/1066.full.pdf

Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.

http://www.cifn.unam.mx/~contrera/bioinfo/papers/5_psipred1999.pdf

Protein secondary structure prediction based on position-specific scoring matrices

Roles moleculaires des proteines de choc thermique dans le fonctionnement des organismes a des temperatures normales et suite a des chocs thermiques; differents genes impliques

The heat-shock proteins

BIOE 402. Medical Technology Assessment. 2 or 3 hours. Bioentrepreneur course. Assessment of medical technology in the context of commercialization. Objectives, competition, market share, funding, pricing, manufacturing, growth, and intellectual property; many issues unique to biomedical products. Course Information: 2 undergraduate hours. 3 graduate hours. Prerequisite(s): Junior standing or above and consent of the instructor.

“Bioinformatics” 특집을 내면서

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

Publisher Summary This chapter discusses the Garnier–Osguthorpe–Robson (GOR) method for predicting protein secondary structure from amino acid sequence. The chapter presents the major principles used by the GOR method and some results obtained with an updated version of this method. The GOR method is one of the most popular of the secondary structure prediction schemes. Through the successive incorporation of observed frequencies of single, then pairs of residues on a local sequence of 17 residues, the accuracy of the GOR method has improved from about 55% up to 64.4%. The GOR method has the advantage over neural network-based methods or nearest-neighbor methods in that it clearly identifies what is taken into account for the prediction and what is neglected. The method provides estimates of probabilities for the three secondary structures at each residue position that can be useful for further application of the method.

GOR method for predicting protein secondary structure from amino acid sequence.

Complementary DNA clones, encoding the LH-hCG (luteinizing hormone-human choriogonadotropic hormone) receptor were isolated by screening a lambda gt11 library with monoclonal antibodies. The primary structure of the protein was deduced from the DNA sequence analysis; the protein contains 696 amino acids with a putative signal peptide of 27 amino acids. Hydropathy analysis suggests the existence of seven transmembrane domains that show homology with the corresponding regions of other G protein-coupled receptors. Three other types of clones corresponding to shorter proteins were observed, in which the putative transmembrane domain was absent. These probably arose through alternative splicing. RNA blot analysis showed similar patterns in testis and ovary with a major RNA of 4700 nucleotides and several minor species. The messenger RNA was expressed in COS-7 cells, yielding a protein that bound hCG with the same affinity as the testicular receptor.

https://science.sciencemag.org/content/sci/245/4917/525.full.pdf

Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain.

Further developments of protein secondary structure prediction using information theory: New parameters and consideration of residue pairs☆

https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2886%2980917-6

Jean Garnier

Papers

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.

GOR method for predicting protein secondary structure from amino acid sequence.

Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain.

Further developments of protein secondary structure prediction using information theory: New parameters and consideration of residue pairs☆

An algorithm for secondary structure determination in proteins based on sequence similarity