J
Jim McWhir
Researcher at The Roslin Institute
Publications - 55
Citations - 11589
Jim McWhir is an academic researcher from The Roslin Institute. The author has contributed to research in topics: Stem cell & Embryonic stem cell. The author has an hindex of 28, co-authored 55 publications receiving 11268 citations. Previous affiliations of Jim McWhir include University of Edinburgh.
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Selective ablation of differentiated cells permits isolation of embryonic stem cell lines from murine embryos with a non-permissive genetic background
Jim McWhir,Angelika Schnieke,Ray Ansell,Helen Wallace,Alan Colman,Ann R. Scott,Alexander Kind +6 more
TL;DR: A strategy to continuously remove differentiated cells by drug selection is described, which generates germline competent ES lines from genotypes that are non-permissive in the absence of selection.
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Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice.
TL;DR: Two consecutive rounds of gene targeting in hypoxanthine phosphoribosyltransferase-deficient feeder-independent murine embryonic stem (ES) cells resulted in a clean exchange of defined DNA fragments with no other DNA remaining at the target locus.
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Gene targeting using a mouse HPRT minigene/HPRT-deficient embryonic stem cell system: inactivation of the mouse ERCC-1 gene.
TL;DR: A convenient system for gene targeting that uses hypoxanthine phosphoribosyltransferase (HPRT) minigenes as the selectable marker in HPRT-deficient mouse embryonic stem (ES) cells is described and the smallerminigenes were found to be as effective as a more conventional marker—the herpes simplex virus thymidine kinase gene.
Journal ArticleDOI
In-Vivo Analysis of Pim-1 Deficiency
Peter W. Laird,N. M. T. Van Der Lugt,Alan Richard Clarke,J. Domen,K. Linders,Jim McWhir,Anton Berns,Martin L. Hooper +7 more
TL;DR: The surprising lack of a readily observed phenotype in the lymphoid compartment of the Pim-1-deficient mice, suggests a heretofore unrecognized degree of in vivo functional redundancy of this highly conserved proto-oncogene.
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Infection efficiency of human and mouse embryonic stem cells using adenoviral and adeno-associated viral vectors.
Joseph R. Smith-Arica,Alison J. Thomson,Ray Ansell,John A. Chiorini,Beverly L. Davidson,Jim McWhir +5 more
TL;DR: Investigation of infection of human and mouse embryonic stem cells with two of the most popular vectors in gene transfer, adenovirus type 5 (Ad5) and adeno-associated virus (AAV; serotypes 2, 4, and 5) shows that Ad5- and AAV2-based vectors are capable of infecting both human andmouse ES cells, in both their undifferentiated and differentiated states.