J
Jim McWhir
Researcher at The Roslin Institute
Publications - 55
Citations - 11589
Jim McWhir is an academic researcher from The Roslin Institute. The author has contributed to research in topics: Stem cell & Embryonic stem cell. The author has an hindex of 28, co-authored 55 publications receiving 11268 citations. Previous affiliations of Jim McWhir include University of Edinburgh.
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DNA microsatellite analysis of Dolly
David Ashworth,Matthew Bishop,Keith H.S. Campbell,Alan Colman,Alex J. Kind,Angelika Schnieke,Sarah C. Blott,H Griffin,Chris Haley,Jim McWhir,Ian Wilmut +10 more
TL;DR: A more detailed microsatellite analysis, which confirms the origin of Dolly, the first animal cloned from an adult mammal, was produced by somatic cell nuclear transfer from a cell population derived from mammary tissue taken from a 6-year-old Finn Dorset ewe.
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Transcriptome alterations due to physiological normoxic (2% O2) culture of human embryonic stem cells
TL;DR: The gene changes associated with 2% O2 culture may be predictive of novel cellular requirements for stable self-renewal, maintenance of pluripotency, and a reduction of hESC-line heterogeneity.
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Pre-selection of integration sites imparts repeatable transgene expression.
TL;DR: Pre-selection of genomic sites for the introduction of transgenes by gene targeting improves the repeatability of transgene expression and provides an efficient means of single copy transGene introduction by homologous recombination.
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Sequential Genetic Modification of the hprt Locus in Human ESCs Combining Gene Targeting and Recombinase-Mediated Cassette Exchange
TL;DR: A double replacement strategy using two sequential gene targeting steps resulting in the targeted correction of an hprt-mutated hESC line is reported.
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Human embryonic stem cells passaged using enzymatic methods retain a normal karyotype and express CD30.
Alison Thomson,Davina Wojtacha,Zoe Hewitt,Helen Priddle,Virginie Sottile,Alex Di Domenico,Judy Fletcher,Martin Waterfall,Néstor López Corrales,Ray Ansell,Jim McWhir +10 more
TL;DR: In all of the TEG, collagenase and EDTA-treated cultures, cells retained consistent telomere length and potentiality, demonstrating that single cell dissociation can be used to maintain karyotypically and phenotypically normal hESCs.