Alexander Kind

TL;DR: In this Letter in the 27 February issue, a production error led to the image for part b of Fig. 1 (fetal fibroblasts) being used twice, as parts b and c.

TL;DR: Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk and produced viable animals by nuclear transfer.

TL;DR: Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.

TL;DR: The development of nuclear-transfer techniques using cultured somatic cells allows animals to be produced without involving germline cells, which enables us to examine the importance of the repair of chromosome ends in the germ line and to test the telomere hypothesis of ageing.

TL;DR: It is shown that α1,3-galactosyltransferase-knockout pig hearts that express human CD46 and thrombomodulin require non-ischaemic preservation with continuous perfusion and post-transplantation growth control to ensure long-term orthotopic function of the xenograft in baboons, the most stringent preclinical xenotransplantation model.