J
Jin Wang
Researcher at Chinese Academy of Sciences
Publications - 45
Citations - 2756
Jin Wang is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Streptomyces coelicolor & Gene. The author has an hindex of 20, co-authored 45 publications receiving 1784 citations. Previous affiliations of Jin Wang include Chinese National Human Genome Center & Shenzhen University.
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Journal ArticleDOI
CRISPR-Cas12a-assisted nucleic acid detection.
Shi-Yuan Li,Qiu-Xiang Cheng,Jingman Wang,Xiao-Yan Li,Zilong Zhang,Song Gao,Rui-Bing Cao,Guoping Zhao,Guoping Zhao,Jin Wang +9 more
TL;DR: In a recent study, it was found that Cas 12a, which belongs to the class 2 type V-A CRISPR-Cas system, performed collateral cleavage on non-targeted ssDNAs upon the formation of the Cas12a/crRNA/target DNA ternary complex.
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CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA.
TL;DR: It is found that only the ternary complex of Cas12a/ crRNA/targeted ssDNA (or targeted dsDNA) was able to cleave the 5′-labelled target ss DNA (target-DNMT1-3-R-FAM5′), and the proposed ssDNA cleavage processes were illustrated.
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cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions
Linchong Sun,Libing Song,Qianfen Wan,Gongwei Wu,Xinghua Li,Yinghui Wang,Jin Wang,Zhaoji Liu,X.C. Zhong,Xiaoping He,Shengqi Shen,Xin Pan,Ai-Ling Li,Yulan Wang,Ping Gao,Huiru Tang,Huafeng Zhang +16 more
TL;DR: The results reveal that aberrant expression of cMyc leads to the enhanced SSP activation, an essential part of metabolic switch, to facilitate cancer progression under nutrient-deprived conditions.
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Characterization of a New GlnR Binding Box in the Promoter of amtB in Streptomyces coelicolor Inferred a PhoP/GlnR Competitive Binding Mechanism for Transcriptional Regulation of amtB
TL;DR: In vivo assays demonstrated that all of the three GlnR binding boxes were required forglnR-mediated activation of amtB transcription under the nitrogen-limited condition, attributable to the PhoP/GlnR counter-regulatory function subjected to further experimental proof.
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Multiplex gene regulation by CRISPR-ddCpf1
Xiaochun Zhang,Xiaochun Zhang,Jingman Wang,Jingman Wang,Qiu-Xiang Cheng,Xuan Zheng,Guoping Zhao,Jin Wang +7 more
TL;DR: It is demonstrated that ddCpf1 alone could be employed for gene repression in Escherichia coli, and the repression was more effective with CRISPR RNAs (crRNAs) specifically targeting to the template strand of its target genes, which was different from that of dCas9.