Joanna Mattis

TL;DR: It is shown that CLARITY enables fine structural analysis of clinical samples, including non-sectioned human tissue from a neuropsychiatric-disease setting, establishing a path for the transmutation of human tissue into a stable, intact and accessible form suitable for probing structural and molecular underpinnings of physiological function and disease.

TL;DR: Subcellular and transcellular trafficking strategies now permit increased potency of optical inhibition without increased light power requirement, and generalizable strategies for targeting cells based not only on genetic identity, but also on morphology and tissue topology, to allow versatile targeting when promoters are not known or in genetically intractable organisms.

TL;DR: This work systematically compared microbial opsins under matched experimental conditions to extract essential principles and identify key parameters for the conduct, design and interpretation of experiments involving optogenetic techniques.

TL;DR: A cation-conducting channelrhodopsin (VChR1) from Volvox carteri that can drive spiking at 589 nm, with excitation maximum red-shifted ∼70 nm compared with ChR2 is described, thereby defining a functionally distinct third category of microbial rhodopin proteins.

TL;DR: It is demonstrated that distinct BNST subregions exert opposite effects in modulating anxiety, separable anxiolytic roles for different anterodorsal BN ST projections are established, and circuit mechanisms underlying selection of features for the assembly of the anxious state are illustrated.