J
Joaquín Castilla
Researcher at Ikerbasque
Publications - 125
Citations - 7823
Joaquín Castilla is an academic researcher from Ikerbasque. The author has contributed to research in topics: Scrapie & Protein Misfolding Cyclic Amplification. The author has an hindex of 43, co-authored 119 publications receiving 7160 citations. Previous affiliations of Joaquín Castilla include United States Department of Agriculture & Scripps Research Institute.
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Journal ArticleDOI
In Vitro Generation of Infectious Scrapie Prions
TL;DR: It is shown that PrPC --> PrPres conversion can be mimicked in vitro by cyclic amplification of protein misfolding, resulting in indefinite amplification of PrPres.
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Caspase‐12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein
TL;DR: It is shown that nanomolar concentrations of purified PrPSc from mouse scrapie brain induce apoptosis of N2A neuroblastoma cells, implicating for the first time the caspase‐12 dependent pathway in a neurodegenerative disease in vivo, and thus offering novel potential targets for the treatment of prion disorders.
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Detection of prions in blood
TL;DR: It is shown that the protein misfolding cyclic amplification (PMCA) technology can be automated and optimized for high-efficiency amplification of PrPSc, and for the first time thatPrPSc has been detected biochemically in blood.
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Ultra-efficient Replication of Infectious Prions by Automated Protein Misfolding Cyclic Amplification
TL;DR: The unprecedented amplification efficiency of PMCA leads to a several billion-fold increase of sensitivity for PrPSc detection as compared with standard tests used to screen prion-infected cattle and at least 4000 times more sensitivity than the animal bioassay.
Journal ArticleDOI
Production of cattle lacking prion protein.
Jiirgen A. Richt,Poothappillai Kasinathan,Amir N. Hamir,Joaquín Castilla,Thillai Sathiyaseelan,Francisco Vargas,Janaki Sathiyaseelan,Hua Wu,Hiroaki Matsushita,Julie Koster,Shinichiro Kato,Isao Ishida,Claudio Soto,James M. Robl,Yoshimi Kuroiwa +14 more
TL;DR: The generation and characterization of PrPC-deficient cattle produced by a sequential gene-targeting system are reported and at over 20 months of age the cattle are clinically, physiologically, histopathologically, immunologically and reproductively normal.