P
Poothappillai Kasinathan
Researcher at Kyowa Hakko Kirin Co., Ltd.
Publications - 23
Citations - 1438
Poothappillai Kasinathan is an academic researcher from Kyowa Hakko Kirin Co., Ltd.. The author has contributed to research in topics: Chromatin & Somatic cell. The author has an hindex of 12, co-authored 23 publications receiving 1370 citations. Previous affiliations of Poothappillai Kasinathan include Westport Innovations.
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Journal ArticleDOI
Cloned transchromosomic calves producing human immunoglobulin
Yoshimi Kuroiwa,Poothappillai Kasinathan,Yoon J. Choi,Rizwan Naeem,Kazuma Tomizuka,Eddie Sullivan,Jason G. Knott,Anae Duteau,Richard A. Goldsby,Barbara A. Osborne,Isao Ishida,James M. Robl +11 more
TL;DR: A human artificial chromosome vector containing the entire unrearranged sequences of the human immunoglobulin heavy-chain (H) and lambda (λ) light-chain loci was introduced into bovine primary fetal fibroblasts using a microcell-mediated chromosome transfer (MMCT) approach.
Journal ArticleDOI
Production of cattle lacking prion protein.
Jiirgen A. Richt,Poothappillai Kasinathan,Amir N. Hamir,Joaquín Castilla,Thillai Sathiyaseelan,Francisco Vargas,Janaki Sathiyaseelan,Hua Wu,Hiroaki Matsushita,Julie Koster,Shinichiro Kato,Isao Ishida,Claudio Soto,James M. Robl,Yoshimi Kuroiwa +14 more
TL;DR: The generation and characterization of PrPC-deficient cattle produced by a sequential gene-targeting system are reported and at over 20 months of age the cattle are clinically, physiologically, histopathologically, immunologically and reproductively normal.
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Sequential targeting of the genes encoding immunoglobulin-mu and prion protein in cattle.
Yoshimi Kuroiwa,Poothappillai Kasinathan,Hiroaki Matsushita,Janaki Sathiyaselan,Eddie Sullivan,Makoto Kakitani,Kazuma Tomizuka,Isao Ishida,James M. Robl +8 more
TL;DR: A sequential gene targeting system for primary fibroblast cells is reported that alleviates the need for germline transmission for complex genetic modifications and should be broadly applicable to gene functional analysis and to biomedical and agricultural applications.
Journal ArticleDOI
Transgenic animal production and animal biotechnology
TL;DR: It is now possible to not only readily make simple insertional genetic modifications, but also to accomplish, more complex, homozygous gene targeting and artificial chromosome transfer in livestock.
Journal ArticleDOI
Antigen-specific human polyclonal antibodies from hyperimmunized cattle
Yoshimi Kuroiwa,Poothappillai Kasinathan,Thillainayagen Sathiyaseelan,Jin-an Jiao,Hiroaki Matsushita,Janaki Sathiyaseelan,Hua Wu,Jenny Mellquist,Melissa Hammitt,Julie Koster,Satoru Kamoda,Katsumi Tachibana,Isao Ishida,James M. Robl +13 more
TL;DR: A Tc bovine system that produces large yields of antigen-specific human polyclonal antibodies and a hIgG-mediated humoral immune response comprising a high proportion of antigen-specific hIGG is reported.