John J. Donnelly

TL;DR: Investigating the role of muscle cells and involvement of professional antigen-presenting cells (APCs) in priming CTL responses following DNA vaccination found expression of antigen by muscle cells in BM chimeric mice after myoblast transplantation is sufficient to induce CTL restricted only by the MHC haplotype of the donor BM, indicating that transfer of antigen from myocytes to professional APCs can occur.

TL;DR: It is demonstrated that transplantation of nucleoprotein (NP)‐transfected myoblasts into syngeneic mice led to the generation of NP‐specific antibodies and CTL, and cross‐strain protective immunity against a lethal challenge with influenza virus, indicating that NP expression by muscle cells after transplantation was sufficient to generate protective cell‐mediated immunity.

TL;DR: The present study characterized in more detail the cellular immune responses induced by NP DNA, which included robust lymphoproliferation and Th1-type cytokine secretion in response to antigen-specific restimulation of splenocytes in vitro.

TL;DR: The studies described below demonstrate that DNA vaccines in reasonable dosages encoding a variety of viral proteins could result in the generation of antibodies, neutralizing antibodies, or cytotoxic T lymphocytes in primates, and these responses could be boosted by repeat administration of the DNA vaccine.

TL;DR: It is shown that GNA2132 induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease, and the protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapped bacterium in human serum.