Jollin A. Shylla

TL;DR: The study provides the molecular characterization based on primary sequence data of the rDNA ITS2 region of the gastrothylacid amphistome flukes and demonstrates the phylogenetic utility of the ITS2 sequence–secondary structure data for inferences at higher taxonomic levels.

TL;DR: In sequence and phylogenetic analyses the COI gene turned out to be the most useful marker in identifying the gastrothylacid species, with the exception of Gastrothylax crumenifer, which showed a high degree of intraspecific variations among its isolates.

TL;DR: A high-throughput sequencing and bioinformatics pipeline for mt genomics for F. buski is described that emphasizes the utility of short read NGS platforms such as Ion Torrent and Illumina in successfully sequencing and assembling the mt genome using innovative approaches for PCR primer design as well as assembly.

TL;DR: 28S (D1–D3) rRNA provided a significant resolution of the taxa corroborating with the taxonomy of these flukes and thus proved to be more robust as a phylogenetic marker for lower levels than 18S rRNA.

TL;DR: The spectrum of FBTs reportedly occurring in the country and having a zoonotic potential is discussed in brief and the tropical climate along with the traditional food practices of various native societies provide a congenial transmission environment for many of these trematodes.