Julian König

TL;DR: Individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) data show that hnRNP C recognizes uridine tracts with a defined long-range spacing consistent with hmRNP particle organization, and integration of transcriptome-wide iCLIP data and alternative splicing profiles into an 'RNA map' indicates how the positioning of hn RNP particles determines their effect on the inclusion of alternative exons.

TL;DR: Using individual nucleotide-resolution ultraviolet cross-linking and immunoprecipitation, it is found that TDP-43 preferentially bound long clusters of UG-rich sequences in vivo, highlighting the importance of T DP-43 for the regulation of splicing in the brain.

TL;DR: In this article, state-of-the-art technologies that can be used to study individual RNA-binding proteins or large complexes such as the ribosome are discussed, and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale.

TL;DR: The improved iCLIP protocol is described and critical optimization and control experiments that are required when applying the method to new RBPs are discussed.

TL;DR: By preventing U2AF65 binding to Alu elements, hnRNP C plays a critical role as a genome-wide sentinel protecting the transcriptome.