Karen Meerovitch

TL;DR: In this paper, a simple assay was developed to determine RNA helicase activity, and it was shown that either eIF-4A or eIF 4B, in combination with eIF4B, exhibits helicase activation.

TL;DR: The results suggest that La protein is involved in poliovirus internal initiation of translation and might function through a similar mechanism in the translation of cellular mRNAs.

TL;DR: In this article, the ability of La protein as well as purified initiation factors, eIF-2, guanine nucleotide exchange factor (GEF), eIF4A, EIF-4B and eIF3, to stimulate the synthesis of P1, the capsid precursor protein, in poliovirus type 1 (Mahoney) RNA-programmed RRL was investigated.

TL;DR: In vitro translational efficiencies of a series of deletion and point mutations within the 5' UTR of poliovirus type 2 (Lansing strain) RNA are determined and it is demonstrated that the 3' border of the core poliov virus ribosome landing pad is located between nucleotides 556 and 585, whereas a region extending between nucleOTides 585 and 612 confers enhanced translation.

TL;DR: It is proposed that NS2/3 processing is a critical step in the viral life cycle and is required to permit the accumulation of sufficient NS3 for RNA replication to occur and it is demonstrated that uncleaved NS 2/3 degradation can be prevented by the addition of a proteasome inhibitor.