Karsten Kuhn

TL;DR: A novel MS/MS-based analysis strategy using isotopomer labels, referred to as "tandem mass tags" (TMTs), for the accurate quantification of peptides and proteins is described, which allows peptides from different samples to be identified by their relative abundance with greater ease and accuracy than other methods.

TL;DR: The validity and robustness of the tandem mass tag (TMT) approach for quantitative MS-based proteomics is demonstrated, and proof of principle studies are carried out using standard protein mixtures and human cerebrospinal fluid (CSF) samples.

TL;DR: This work serves as a blueprint for expanding the multiplexing capacity of the TMT reagents to at least 10-plex and possibly up to 18-plex, and is able to quantify across eight samples simultaneously by combining the (13)C- and (15)N-containing reporter ions.

TL;DR: A set of isobaric labeling reagents called TMTpro enables deep quantitative comparisons of proteome measurements across 16 samples, and identifies and dose-stratified staurosporine binding to 228 cellular kinases in just one, 18-h experiment.

TL;DR: The ability to pool 16 samples reduces the overall amount of sample required for each channel and it is anticipated that TMTpro reagents will be a useful enhancement for any protocol that benefits from sample pooling and should reduce missing data.