K
Keisuke Shima
Researcher at Shimadzu Corp.
Publications - 22
Citations - 284
Keisuke Shima is an academic researcher from Shimadzu Corp.. The author has contributed to research in topics: Mass spectrometry & Polyacrylamide gel electrophoresis. The author has an hindex of 9, co-authored 21 publications receiving 230 citations.
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Journal ArticleDOI
Heat-shock protein 27 (Hsp27) as a target of methylglyoxal in gastrointestinal cancer
Tomoko Oya-Ito,Yuji Naito,Tomohisa Takagi,Osamu Handa,Hirofumi Matsui,Masaki Yamada,Keisuke Shima,Toshikazu Yoshikawa +7 more
TL;DR: The transfer of methylglyoxal-modified heat-shock protein 27 into rat intestinal epithelial cell line RIE was even more effective in preventing apoptotic cell death than that of native control heat- shock protein 27 and protected the cells against both the hydrogen peroxide- and cytochrome c-mediated caspase activation, and the hydrogenperoxide-induced production of intracellular reactive oxygen species.
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A simple and highly successful C‐terminal sequence analysis of proteins by mass spectrometry
Hiroki Kuyama,Keisuke Shima,Kazuhiro Sonomura,Minoru Yamaguchi,Eiji Ando,Osamu Nishimura,Osamu Nishimura,Susumu Tsunasawa,Susumu Tsunasawa +8 more
TL;DR: A highly successful, de novo C‐terminal sequencing method of proteins by employing succinimidyloxycarbonylmethyl tris (2,4,6‐trimethoxyphenyl) phosphonium bromide and mass spectrometry is described.
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PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry.
Ayako Takemori,David S Butcher,Victoria M. Harman,Philip Brownridge,Keisuke Shima,Daisuke Higo,Jun Ishizaki,Hitoshi Hasegawa,Junpei Suzuki,Masakatsu Yamashita,Joseph A. Loo,Rachel R. Ogorzalek Loo,Robert J. Beynon,Lissa C. Anderson,Nobuaki Takemori +14 more
TL;DR: A novel experimental workflow, called Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS ('PEPPI-MS'), which allows top-down MS of PAGE-separated proteins and will be a powerful prefractionation strategy for top- down proteomics.
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Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.
Yuka Yajima,Takuya Hiratsuka,Yu Kakimoto,Shuichiro Ogawa,Keisuke Shima,Yuzo Yamazaki,Kenichi Yoshikawa,Keiji Tamaki,Tatsuaki Tsuruyama +8 more
TL;DR: ROI analysis using MALDI-IMS may be a promising technique for the identification of biomarkers for pathological studies that involve the comparison of diseased and control areas.
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Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids.
Minoru Yamaguchi,Daisuke Nakayama,Keisuke Shima,Hiroki Kuyama,Eiji Ando,Taka-aki Okamura,Norikazu Ueyama,Takashi Nakazawa,Shigemi Norioka,Osamu Nishimura,Osamu Nishimura,Susumu Tsunasawa,Susumu Tsunasawa +12 more
TL;DR: A universal method for N-terminal sequence analysis of proteins is proposed, employing DITC resins instead of avidin resins used in the previous method, and it has been possible to isolate selectively N-Terminal peptides from proteins regardless of modification of N- terminal amino acids.