K
Kelly M. Colvin
Researcher at University of Washington
Publications - 8
Citations - 1195
Kelly M. Colvin is an academic researcher from University of Washington. The author has contributed to research in topics: Deacetylase activity & Biofilm matrix. The author has an hindex of 6, co-authored 8 publications receiving 980 citations.
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Journal ArticleDOI
The Pel Polysaccharide Can Serve a Structural and Protective Role in the Biofilm Matrix of Pseudomonas aeruginosa
Kelly M. Colvin,Vernita Gordon,Keiji Murakami,Bradley R. Borlee,Daniel J. Wozniak,Gerard C. L. Wong,Matthew R. Parsek +6 more
TL;DR: It is shown that expression of the pel gene cluster and PelF protein levels are enhanced during biofilm growth compared to liquid cultures, suggesting that Pel is capable of playing both a structural and a protective role in P. aeruginosa biofilms.
Journal ArticleDOI
The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix
Kelly M. Colvin,Yasuhiko Irie,Catherine S. Tart,Rodolfo Urbano,John C. Whitney,Cynthia Ryder,P. Lynne Howell,Daniel J. Wozniak,Matthew R. Parsek +8 more
TL;DR: This study evaluated a range of clinical and environmental P.aeruginosa isolates for their dependence on Pel and Psl for biofilm development and revealed both unique and redundant roles for two distinct biofilm exopolysaccharides.
Journal ArticleDOI
Structure of the Cytoplasmic Region of PelD, a Degenerate Diguanylate Cyclase Receptor That Regulates Exopolysaccharide Production in Pseudomonas aeruginosa
John C. Whitney,Kelly M. Colvin,Lindsey S. Marmont,Howard Robinson,Matthew R. Parsek,P. Lynne Howell +5 more
TL;DR: It is demonstrated that PelD binds c-di-GMP with low micromolar affinity and that mutation of residues involved in binding not only decreases the affinity of this interaction but also abrogates PEL-specific phenotypes in vivo.
Journal ArticleDOI
PelA Deacetylase Activity Is Required for Pel Polysaccharide Synthesis in Pseudomonas aeruginosa
Kelly M. Colvin,Noor Alnabelseya,Perrin Baker,John C. Whitney,P. Lynne Howell,Matthew R. Parsek +5 more
TL;DR: The deacetylase activity of PelA is important for the production of the Pel polysaccharide, and results suggest that these mutants were deficient in Pel-dependent biofilm formation and wrinkly colony morphology in vivo.
Book ChapterDOI
Different Methods for Culturing Biofilms In Vitro
S. Brook Peterson,Yasuhiko Irie,Bradley R. Borlee,Keiji Murakami,Joe J. Harrison,Kelly M. Colvin,Matthew R. Parsek +6 more
TL;DR: The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved because of the lack of standardized methods for culturing biofilm communities.