K
Keiji Murakami
Researcher at University of Tokushima
Publications - 57
Citations - 2788
Keiji Murakami is an academic researcher from University of Tokushima. The author has contributed to research in topics: Pseudomonas aeruginosa & Biofilm. The author has an hindex of 23, co-authored 52 publications receiving 2318 citations. Previous affiliations of Keiji Murakami include University of Washington.
Papers
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Journal ArticleDOI
The Pel Polysaccharide Can Serve a Structural and Protective Role in the Biofilm Matrix of Pseudomonas aeruginosa
Kelly M. Colvin,Vernita Gordon,Keiji Murakami,Bradley R. Borlee,Daniel J. Wozniak,Gerard C. L. Wong,Matthew R. Parsek +6 more
TL;DR: It is shown that expression of the pel gene cluster and PelF protein levels are enhanced during biofilm growth compared to liquid cultures, suggesting that Pel is capable of playing both a structural and a protective role in P. aeruginosa biofilms.
Journal ArticleDOI
Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix
Bradley R. Borlee,Aaron David Goldman,Keiji Murakami,Ram Samudrala,Daniel J. Wozniak,Matthew R. Parsek +5 more
TL;DR: Psl‐specific lectin staining suggests that CdrA either cross‐links Psl polysaccharide polymers and/or tethers Psl to the cells, resulting in increased biofilm structural stability, and this study identifies a key protein structural component of the P. aeruginosa EPS matrix.
Journal ArticleDOI
Fluorescence-Based Reporter for Gauging Cyclic Di-GMP Levels in Pseudomonas aeruginosa
Morten Rybtke,Bradley R. Borlee,Keiji Murakami,Yasuhiko Irie,Morten Hentzer,Thomas E. Nielsen,Thomas E. Nielsen,Michael Givskov,Michael Givskov,Matthew R. Parsek,Tim Tolker-Nielsen +10 more
TL;DR: Fluorescent monitors that can gauge the cellular level of cyclic di-GMP in P. aeruginosa and display a significant potential for use in the identification of novel antipathogenic compounds targeting cyclicDiGMP signaling are described.
Journal ArticleDOI
The FleQ protein from Pseudomonas aeruginosa functions as both a repressor and an activator to control gene expression from the pel operon promoter in response to c-di-GMP
TL;DR: Analysis of wild type and mutated versions of pelA-lacZ transcriptional fusions suggests that FleQ represses gene expression from box 2 and activates gene expression in response to c-di-GMP from box 1.
Journal ArticleDOI
Heterogeneity in surface sensing suggests a division of labor in Pseudomonas aeruginosa populations.
Catherine R. Armbruster,Calvin K. Lee,Jessica Parker-Gilham,Jaime de Anda,Aiguo Xia,Kun Zhao,Keiji Murakami,Boo Shan Tseng,Lucas R. Hoffman,Fan Jin,Fan Jin,Caroline S. Harwood,Gerard C. L. Wong,Matthew R. Parsek,Matthew R. Parsek +14 more
TL;DR: This work describes how the Wsp system generates heterogeneity in surface sensing, resulting in two physiologically distinct subpopulations of cells, and shows that this heterogeneity strongly correlates to surface behavior for descendent cells.