Book ChapterDOI
Different Methods for Culturing Biofilms In Vitro
S. Brook Peterson,Yasuhiko Irie,Bradley R. Borlee,Keiji Murakami,Joe J. Harrison,Kelly M. Colvin,Matthew R. Parsek +6 more
- pp 251-266
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TLDR
The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved because of the lack of standardized methods for culturing biofilm communities.Abstract:
The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved. One of the underlying reasons is the lack of standardized methods for culturing biofilm communities. Many times, the culturing format will be unique to the study in question, resulting in difficulties when other labs attempt to confirm results produced by another lab. Another issue has been the limited utility of different culturing methods for the specific research questions being asked. For example, culturing formats designed to be accessible to microscopy are not always suited for other types of analyses, such as harvesting biofilm biomass for biochemical measurements.read more
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Journal ArticleDOI
Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteria
Clayton W. Hall,Thien-Fah Mah +1 more
TL;DR: This review summarises both historical and recent scientific data in support of the known biofilm resistance and tolerance mechanisms and suggestions for future work in the field are provided.
Journal ArticleDOI
Critical review on biofilm methods
Joana Azeredo,Nuno F. Azevedo,Romain Briandet,Nuno Cerca,Tom Coenye,Ana Rita Costa,Mickaël Desvaux,Giovanni Di Bonaventura,Michel Hébraud,Zoran Jaglic,Miroslava Kačániová,Susanne Knøchel,Anália Lourenço,Filipe Mergulhão,Rikke Louise Meyer,George Nychas,Manuel Simões,Odile Tresse,Claus Sternberg +18 more
TL;DR: This review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms by giving a critical perspective, highlighting the advantages and limitations of several methods.
Journal ArticleDOI
Options and Limitations in Clinical Investigation of Bacterial Biofilms
Maria Magana,Christina Sereti,Anastasios Ioannidis,Courtney A. Mitchell,Anthony R. Ball,Emmanouil Magiorkinis,Stylianos Chatzipanagiotou,Michael R. Hamblin,Michael R. Hamblin,Maria Hadjifrangiskou,George P. Tegos +10 more
TL;DR: A synopsis of the methodological landscape of biofilm analysis is provided, including an evaluation of the current trends in methodological research, and recommendations defining the most appropriate methodological tools for clinical settings are made.
Journal ArticleDOI
Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa
Dorit Cohen,Undine Mechold,Hadas Nevenzal,Yafit Yarmiyhu,Trevor E. Randall,Denice C. Bay,Jacquelyn D. Rich,Matthew R. Parsek,Volkhard Kaever,Joe J. Harrison,Ehud Banin +10 more
TL;DR: Olin—a 3ʹ→5ʹ exonuclease highly conserved among Actinobacteria, Beta-, Delta- and Gammaproteobacteria—is identified as the primary enzyme responsible for pGpG degradation in Pseudomonas aeruginosa cells, revealing that nanoribonucleases, which have been considered housekeeping proteins crucial for mRNA turnover, also have a key role in c-di-GMP signaling.
Journal ArticleDOI
Approaches to biofilm-associated infections: the need for standardized and relevant biofilm methods for clinical applications
TL;DR: This review will explore the issues that need to be considered in developing performance standards for anti-biofilm therapeutics and provide a rationale for the need to standardize models/methods that are clinically relevant.
References
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Journal ArticleDOI
Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
George A. O'Toole,Roberto Kolter +1 more
TL;DR: The isolation and characterization of mutants of Pseudomonas aeruginosa PA14 defective in the initiation of biofilm formation on an abiotic surface, polyvinylchloride (PVC) plastic are reported and evidence that microcolonies form by aggregation of cells present in the monolayer is presented.
Journal ArticleDOI
Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis
George A. O'Toole,Roberto Kolter +1 more
TL;DR: The genetic analyses suggest that biofilm formation can proceed via multiple, convergent signalling pathways, which are regulated by various environmental signals, and that of the 24 sad mutants analysed in this study, only three had defects in genes of known function.
Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Physiological heterogeneity in biofilms.
TL;DR: The processes that generate chemical gradients inBiofilms, the genetic and physiological responses of the bacteria as they adapt to these gradients and the techniques that can be used to visualize and measure the microscale physiological heterogeneities of bacteria in biofilms are discussed.
Journal ArticleDOI
Quantification of biofilm structures by the novel computer program COMSTAT.
Arne Heydorn,Alex Toftgaard Nielsen,Morten Hentzer,Claus Sternberg,Michael Givskov,Bjarne Kjær Ersbøll,Søren Molin +6 more
TL;DR: Analysis of biofilms of P. aureofaciens growing on 0.03 mM, 0.1 mM or 0.5 mM citrate minimal media showed that mean biofilm thickness increased with increasing citrate concentration, whereas surface to volume ratio increased with higher citrate concentrations.