Kelly M. Colvin

TL;DR: It is shown that expression of the pel gene cluster and PelF protein levels are enhanced during biofilm growth compared to liquid cultures, suggesting that Pel is capable of playing both a structural and a protective role in P. aeruginosa biofilms.

TL;DR: This study evaluated a range of clinical and environmental P.aeruginosa isolates for their dependence on Pel and Psl for biofilm development and revealed both unique and redundant roles for two distinct biofilm exopolysaccharides.

TL;DR: It is demonstrated that PelD binds c-di-GMP with low micromolar affinity and that mutation of residues involved in binding not only decreases the affinity of this interaction but also abrogates PEL-specific phenotypes in vivo.

TL;DR: The deacetylase activity of PelA is important for the production of the Pel polysaccharide, and results suggest that these mutants were deficient in Pel-dependent biofilm formation and wrinkly colony morphology in vivo.

TL;DR: The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved because of the lack of standardized methods for culturing biofilm communities.