Kelvin Y. Chen

TL;DR: ASAP-seq as discussed by the authors uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing.

TL;DR: In conclusion, inhibition of the CD28-PKC-NF-κB signaling pathway in iTreg generation enables de novo acquisition of Treg-specific DNA hypomethylation at Treg signature genes and abundant production of functionally stable antigen-specific iTregs for therapeutic purposes.

TL;DR: In this paper, the authors show that hierarchical and coordinated activation of Foxp3 CNS0 and CNS3 initiates and stabilizes gene expression, thereby crucially controlling Treg cell development, maintenance, and consequently immunological self-tolerance.

TL;DR: The utility of ASAP-seq is demonstrated by revealing coordinated and distinct changes in chromatin, RNA, and surface proteins during native hematopoietic differentiation, peripheral blood mononuclear cell stimulation, and as a combinatorial decoder and reporter of multiplexed perturbations in primary T cells.