Kevin Hua

TL;DR: This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.

TL;DR: It is reported that ZFNs can be engineered to induce a site-specific DNA single-strand break (SSB) or nick and the potential for a SSB to direct repair pathway choice may prove advantageous for certain therapeutic applications such as the targeted correction of human disease-causing mutations.

TL;DR: Evidence is provided that ZFN-mediated disruption of genomic cxcr4 provides a selective advantage to CD4(+) T cells during HIV-1 infection, which is found to be stable, conferred resistance, and provided for continued cell enrichment during AIDS infection.

TL;DR: This chapter will provide an overview of the current knowledge of PDAC pathogenesis at the genetic and molecular level as well as novel therapeutic opportunities to treat this highly aggressive disease.

TL;DR: The C34-conjugated coreceptors were expressed on the surface of T cell lines and primary CD4 T cells, retained the ability to mediate chemotaxis in response to cognate chemokines, and were highly resistant to HIV-1 utilization for entry.