Ki Bang Song

TL;DR: Levan, fructo-oligosaccharides and fructosyl derivatives were formed from sucrose using recombinant levansucrase from Rahnella aquatilis, resulting in 57% of the theoretical yield and the increase of levan and oligosACcharides formation was not achieved by adding water-miscible organic solvents.

TL;DR: Immobilized levansucrase retained 61% of the original activity after five repeated uses of immobilization and showed the less polymerizing activity in levan formation, producing the low-molecular weight (MW) levan.

TL;DR: The induced cell culture of recombinant E. coli could grow up to a much higher cell concentration with continuing recombinant enzyme synthesis, and in the case of the controlled feed of lactose, the maximum activities of total and extracellular LFTase were nearly 100% and 70% higher, respectively.

TL;DR: Secretion of levansucrase from Zymomonas mobilis in Escherichiacoli by glycine supplement was investigated and a significant amount of levanucrase (about 25% of total activity) was found in intact whole-cells.

TL;DR: Methyl β-D-fructoside(MF) was formed from sucrose and methanol by a transfructosylation reaction using recombinant levansucrase from Rahnella aquatilis as mentioned in this paper.