L
Larissa M. Podust
Researcher at University of Montana
Publications - 93
Citations - 4751
Larissa M. Podust is an academic researcher from University of Montana. The author has contributed to research in topics: Active site & Trypanosoma cruzi. The author has an hindex of 41, co-authored 86 publications receiving 4258 citations. Previous affiliations of Larissa M. Podust include University of Zurich & University of California, San Francisco.
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Journal ArticleDOI
Crystal structure of cytochrome P450 14α-sterol demethylase (CYP51) from Mycobacterium tuberculosis in complex with azole inhibitors
TL;DR: Mapping mutations identified in Candida albicans azole-resistant isolates indicates that azole resistance in fungi develops in protein regions involved in orchestrating passage of CYP51 through different conformational stages along the catalytic cycle rather than in residues directly contacting fluconazole.
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Diversity of P450 enzymes in the biosynthesis of natural products.
TL;DR: Engineering microbial-derived P450 enzymes to accommodate alternative substrates and add new functions continues to be an important near- and long-term practical goal driving the structural characterization of these molecules.
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Two approaches to discovering and developing new drugs for Chagas disease
James H. McKerrow,Patricia S. Doyle,Juan C. Engel,Larissa M. Podust,Stephanie A. Robertson,Rafaela Salgado Ferreira,T Saxton,Michelle R. Arkin,Iain D. Kerr,Linda S. Brinen,Charles S. Craik +10 more
TL;DR: This review will focus on two general approaches carried out at the Sandler Center, University of California, San Francisco, to address the challenge of developing new drugs for the treatment of Chagas disease.
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Binding of Two Flaviolin Substrate Molecules, Oxidative Coupling, and Crystal Structure of Streptomyces coelicolor A3(2) Cytochrome P450 158A2
Bin Zhao,F. Peter Guengerich,Aouatef Bellamine,David C. Lamb,Miho Izumikawa,Li Lei,Larissa M. Podust,Munirathinam Sundaramoorthy,John A. Kalaitzis,L. Manmohan Reddy,Steven L. Kelly,Bradley S. Moore,Donald F. Stec,Markus Voehler,John R. Falck,Tsutomu Shimada,Michael R. Waterman +16 more
TL;DR: Following cloning, expression, and purification of this cytochrome P450, it is shown that it can produce dimer and trimer products from the substrate flaviolin and that the structures of two of the dimeric products were established using mass spectrometry and multiple NMR methods.
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The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae
David H. Sherman,Shengying Li,Liudmila V. Yermalitskaya,Youngchang Kim,Jarrod A. Smith,Michael R. Waterman,Larissa M. Podust +6 more
TL;DR: Four x-ray crystal structures and allied functional studies for PikC, the remarkable P450 monooxygenase responsible for production of a number of related macrolide products from the Pik pathway, reveal that PikC substrate tolerance and product diversity result from a combination of alternative anchoring modes rather than an induced fit mechanism.