Luigi Warren

TL;DR: It is shown that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols and represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling, and regenerative medicine.

TL;DR: A systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based “digital RT-PCR” assay that can count template molecules in cDNA samples prepared from single cells is reported, which demonstrates caveats of normalizing expression data to endogenous controls and underscores the need to put gene measurement on an absolute, copy-per-cell basis.

TL;DR: In this paper, a feeder-free, xeno-free protocol for mRNA-based iPSC derivation is presented. But, this protocol requires the use of feeder cells that add complexity and variability to the procedure while introducing a route for contamination with non-human-derived biological material.

TL;DR: In this paper, the authors describe methods, compositions, and kits comprising synthetic, modified RNAs for changing the phenotype of a cell or cells, such as expressing a polypeptide or altering the developmental potential.

TL;DR: With age, HSCs show an increased propensity to differentiate towards myeloid rather than lymphoid lineages, which may contribute to the decline in lymphopoiesis that attends aging.