Derrick J. Rossi

TL;DR: It is shown that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols and represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling, and regenerative medicine.

TL;DR: It is shown that 22 human induced pluripotent stem (hiPS) cell lines reprogrammed using five different methods each contained an average of five protein-coding point mutations in the regions sampled, and that hiPS cells acquire genetic modifications in addition to epigenetic modifications.

TL;DR: Although deficiencies in several genomic maintenance pathways did not deplete stem cell reserves with age, stem cell functional capacity was severely affected under conditions of stress, leading to loss of reconstitution and proliferative potential, diminished self-renewal, increased apoptosis and, ultimately, functional exhaustion.

TL;DR: Estimation of cell intrinsic functional and molecular properties of highly purified long-term hematopoietic stem cells from young and old mice found that LT-HSC aging was accompanied by cell autonomous changes, including increased stem cell self-renewal, differential capacity to generate committed myeloid and lymphoid progenitors, and diminished lymphoid potential.

TL;DR: Evidence linking stem cell dysfunction to the pathophysiological conditions accompanying aging is examined, focusing on the mechanisms underlying stem cell decline and their contribution to disease pathogenesis.