L
Luis G.C. Pacheco
Researcher at Federal University of Bahia
Publications - 72
Citations - 1699
Luis G.C. Pacheco is an academic researcher from Federal University of Bahia. The author has contributed to research in topics: Corynebacterium pseudotuberculosis & Caseous lymphadenitis. The author has an hindex of 19, co-authored 62 publications receiving 1371 citations. Previous affiliations of Luis G.C. Pacheco include Universidade Federal de Minas Gerais & Brigham and Women's Hospital.
Papers
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Journal ArticleDOI
Immunomodulatory properties of Schistosoma mansoni proteins Sm200 and SmKI-1 in vitro and in a murine model of allergy to the mite Blomia tropicalis.
Camile L. S. Alves,Leonardo Freire Santiago,Marina B. R. Santana,Barbara C. Figueiredo,Suellen B. Morais,Sergio C. Oliveira,Luis G.C. Pacheco,Neuza Maria Alcantara-Neves,Carina S. Pinheiro +8 more
TL;DR: Evaluated recombinant S. mansoni proteins Sm200, and SmKI-1 to induce immunomodulation in vitro, using peripheral blood mononuclear cells from atopic and non-atopic individuals, and in vivo, in a murine model of allergy to the mite B. tropicalis.
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High-Quality Resolution of the Outbreak-Related Zika Virus Genome and Discovery of New Viruses Using Ion Torrent-Based Metatranscriptomics.
Silvia Ines Sardi,Rejane Hughes Carvalho,Luis G.C. Pacheco,João Paulo Pereira de Almeida,Emília Maria Medeiros de Andrade Belitardo,Carina S. Pinheiro,Gubio Soares Campos,Eric R.G.R. Aguiar,Eric R.G.R. Aguiar +8 more
TL;DR: This work used RNA-based metatranscriptomics associated with Ion Torrent deep sequencing to allow for the high-quality reconstitution of an outbreak-related Zika virus (ZIKV) genome (10,739 nt), with extended 5'-UTR and 3-UTR regions, using a newly-implemented bioinformatics approach.
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In vivo cleavage of solubility tags as a tool to enhance the levels of soluble recombinant proteins in Escherichia coli.
Filipe S R Silva,Sara Tereza Lisboa dos Santos,Roberto Meyer,Eduardo Santos da Silva,Carina Da Silva Pinheiro,Neuza Maria Alcantara-Neves,Luis G.C. Pacheco +6 more
TL;DR: In this article, the authors present a review of different approaches for controlled intracellular processing systems tailored to produce soluble untagged proteins in E. coli, through co-expression of a site-specific protease, simplify the recombinant protein purification process, and promote the screening of molecules that fail to remain soluble after tag removal.
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Single-Input Regulatory Cascade for in vivo Removal of the Solubility Tag in Fusion Recombinant Proteins Produced by Escherichia coli
Filipe S R Silva,Sara Patrícia de Oliveira Santos,Roberto Meyer,Neuza Maria Alcantara-Neves,Carina S. Pinheiro,Luis G.C. Pacheco +5 more
TL;DR: A synthetic biology-based strategy to permit in vivo removal of a solubility tag through co-expression of the fusion recombinant protein (EDA-EGFP) and the tag-cleaving protease (TEVp), in a controlled manner is described.
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Corynebacterium phoceense - a rare Corynebacterium species isolated from a urine sample
Claudia Barberis,German Matias Traglia,Marisa Almuzara,Danilo J.P.G. Rocha,Carolina S. Santos,Eric R.G.R. Aguiar,Luis G.C. Pacheco,Carlos Vay +7 more
TL;DR: A new episode of urinary tract infection caused by Corynebacterium phoceense is document and the whole-genome sequencing, phenotypic characteristics and mass spectra obtained by matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) are described.