Showing papers by "Manfred Lindau published in 2019"
Max Planck Society1, Stanford University2, University of Virginia3, University of Wisconsin-Madison4, University of Cincinnati Academic Health Center5, Princeton University6, Cornell University7, Huazhong University of Science and Technology8, University of Texas Southwestern Medical Center9, Iowa State University10, Heidelberg University11, Seoul National University12, Yale University13
TL;DR: Researchers working on synaptic vesicle fusion and neurotransmitter release share their views on the most interesting developments in their field and the challenges that lie ahead.
Abstract: Researchers working on synaptic vesicle fusion and neurotransmitter release share their views on the most interesting developments in their field and the challenges that lie ahead.
19 citations
TL;DR: It is shown that the antidepressant drug bupropion significantly increases the amount of neurotransmitter released in individual quantal release events and citalopram accelerates rapid neurotransmitter release following stimulation and follow‐up patch‐clamp experiments reveal that this is because of the increase in the pool of readily releasable vesicles.
Abstract: Neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease, and Huntington's disease, are considered incurable and significantly reduce the quality of life of the patients. A variety of drugs that modulate neurotransmitter levels have been used for the treatment of the neurodegenerative diseases but with limited efficacy. In this work, an amperometric complementary metal-oxide-semiconductor (CMOS) chip is used for high-throughput drug testing with respect to the modulation of transmitter release from single vesicles using chromaffin cells prepared from bovine adrenal glands as a model system. Single chromaffin cell amperometry was performed with high efficiency on the surface-modified CMOS chip and follow-up whole-cell patch-clamp experiments were performed to determine the readily releasable pool sizes. We show that the antidepressant drug bupropion significantly increases the amount of neurotransmitter released in individual quantal release events. The antidepressant drug citalopram accelerates rapid neurotransmitter release following stimulation and follow-up patch-clamp experiments reveal that this is because of the increase in the pool of readily releasable vesicles. These results shed light on the mechanisms by which bupropion and citalopram may be potentially effective in the treatment of neurodegenerative diseases. These results demonstrate that the CMOS amperometry chip technology is an excellent tool for drug testing to determine the specific mechanisms by which they modulate neurotransmitter release.
6 citations
TL;DR: The event correlation microscopy method provides time superresolution beyond the photon rate limit and imaging frame duration with well-defined precision, providing guidelines for camera exposure time settings depending on imaging signal properties and camera parameters for optimal time resolution.
1 citations