M
Manuel Serif
Researcher at University of Konstanz
Publications - 13
Citations - 384
Manuel Serif is an academic researcher from University of Konstanz. The author has contributed to research in topics: Phaeodactylum tricornutum & Thylakoid. The author has an hindex of 7, co-authored 12 publications receiving 241 citations. Previous affiliations of Manuel Serif include Norwegian University of Science and Technology & Scottish Association for Marine Science.
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Journal ArticleDOI
One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing.
Manuel Serif,Gwendoline Dubois,Gwendoline Dubois,Gwendoline Dubois,Anne-Laure Finoux,Anne-Laure Finoux,Anne-Laure Finoux,Marie-Ange Teste,Marie-Ange Teste,Marie-Ange Teste,Denis Jallet,Denis Jallet,Denis Jallet,Fayza Daboussi,Fayza Daboussi,Fayza Daboussi +15 more
TL;DR: A highly efficient multiplex genome-editing method in the diatom Phaeodactylum tricornutum, relying on the biolistic delivery of CRISPR-Cas9 ribonucleoproteins coupled with the identification of two endogenous counter-selectable markers, PtUMPS and PtAPT is reported.
Journal ArticleDOI
A fast and reliable strategy to generate TALEN-mediated gene knockouts in the diatom Phaeodactylum tricornutum
TL;DR: A cost-effective TALEN generation system previously established for mammalian cells for the use in P. tricornutum appears to be highly efficient: targeted mutation events were detected in 50% of all transformants obtained, whereas 21% of the transformants were found to be bi-allelic knockout lines.
Journal ArticleDOI
Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum
Jochen M. Buck,Jonathan Sherman,Carolina Río Bártulos,Manuel Serif,Marc Halder,Jan Henkel,Jan Henkel,Angela Falciatore,Johann Lavaud,Maxim Y. Gorbunov,Peter G. Kroth,Paul G. Falkowski,Bernard Lepetit +12 more
TL;DR: It is shown that induced thermal dissipation in the diatom Phaeodactylum tricornutum Pt4 is Lhcx protein-dependent and correlates with a reduced functional absorption cross-section of photosystem II.
Journal ArticleDOI
Genome editing in diatoms: achievements and goals
Peter G. Kroth,Atle M. Bones,Fayza Daboussi,Maria Immacolata Ferrante,Marianne Jaubert,Misha Kolot,Misha Kolot,Marianne Nymark,Carolina Río Bártulos,Andrés Ritter,Monia Teresa Russo,Manuel Serif,Per Winge,Angela Falciatore +13 more
TL;DR: An overview of the genetic toolbox that is currently available for performing stable genetic modifications in diatoms is provided and novel challenges that need to be addressed are discussed to fully exploit the potential of these technologies for the characterization of diatom biology and for metabolic engineering.
Journal ArticleDOI
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes
Ankan Banerjee,Elena Herman,Manuel Serif,Manuel Maestre-Reyna,Sebastian Hepp,Richard Pokorny,Peter G. Kroth,Lars-Oliver Essen,Tilman Kottke +8 more
TL;DR: It is proposed that aureochrome signaling relies on an allosteric pathway from LOV to bZIP that results in conformational changes near the bZip-DNA interface without major effects on the binding affinity.