G
Gwendoline Dubois
Researcher at University of Toulouse
Publications - 9
Citations - 705
Gwendoline Dubois is an academic researcher from University of Toulouse. The author has contributed to research in topics: Transcription activator-like effector nuclease & Genome editing. The author has an hindex of 6, co-authored 8 publications receiving 566 citations. Previous affiliations of Gwendoline Dubois include University of Copenhagen & Centre national de la recherche scientifique.
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Journal ArticleDOI
Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology
Fayza Daboussi,Sophie Leduc,Alan Maréchal,Gwendoline Dubois,Valérie Guyot,Christophe Perez-Michaut,Alberto Amato,Angela Falciatore,Angela Falciatore,Alexandre Juillerat,Marine Beurdeley,Daniel F. Voytas,Laurent Cavarec,Philippe Duchateau +13 more
TL;DR: The generation of an enhanced lipid-producing strain through the disruption of the UDP-glucose pyrophosphorylase gene exemplifies the power of genome engineering to harness diatoms for biofuel production.
Journal ArticleDOI
One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing.
Manuel Serif,Gwendoline Dubois,Gwendoline Dubois,Gwendoline Dubois,Anne-Laure Finoux,Anne-Laure Finoux,Anne-Laure Finoux,Marie-Ange Teste,Marie-Ange Teste,Marie-Ange Teste,Denis Jallet,Denis Jallet,Denis Jallet,Fayza Daboussi,Fayza Daboussi,Fayza Daboussi +15 more
TL;DR: A highly efficient multiplex genome-editing method in the diatom Phaeodactylum tricornutum, relying on the biolistic delivery of CRISPR-Cas9 ribonucleoproteins coupled with the identification of two endogenous counter-selectable markers, PtUMPS and PtAPT is reported.
Journal ArticleDOI
Comprehensive analysis of the specificity of transcription activator-like effector nucleases
Alexandre Juillerat,Gwendoline Dubois,Julien Valton,Séverine Thomas,Stefano Stella,Alan Maréchal,Stéphanie Langevin,Nassima Benomari,Claudia Bertonati,George H. Silva,Fayza Daboussi,Jean-Charles Epinat,Guillermo Montoya,Aymeric Duclert,Philippe Duchateau +14 more
TL;DR: It is reported that TALEN can only accommodate a relatively small number of position-dependent mismatches while maintaining a detectable activity at endogenous loci in vivo, demonstrating the high specificity of these molecular tools.
Journal ArticleDOI
Production of Medium Chain Fatty Acids by Yarrowia lipolytica: Combining Molecular Design and TALEN to Engineer the Fatty Acid Synthase
Coraline Rigouin,Marc Guéroult,Christian Croux,Gwendoline Dubois,Vinciane Borsenberger,Sophie Barbe,Alain Marty,Fayza Daboussi,Isabelle André,Florence Bordes +9 more
TL;DR: TALEN (transcription activator-like effector nucleases)-based genome editing technology was applied for the first time to Yarrowia lipolytica and proved to be very efficient for inducing targeted genome modifications.
Journal ArticleDOI
BurrH: a new modular DNA binding protein for genome engineering
Alexandre Juillerat,Claudia Bertonati,Gwendoline Dubois,Valérie Guyot,Séverine Thomas,Julien Valton,Marine Beurdeley,George H. Silva,Fayza Daboussi,Philippe Duchateau +9 more
TL;DR: BurgerH as discussed by the authors is a new DNA binding protein from Burkholderia rhizoxinica that is composed of highly polymorphic DNA targeting modules and can be used to efficiently induce in vivo targeted mutagenesis and targeted gene insertion at a desired locus.