Matthew Mayho

TL;DR: Saturation-scale mutagenesis allows prioritization of intervention targets in the genome of the most important cause of malaria, and confirms the proteasome-degradation pathway is a high-value druggable target.

TL;DR: An optimized high-yield library preparation and sequencing protocol for TraDIS experiments and a novel software pipeline for analysis of the resulting data are described.

TL;DR: The development of a method for rapidly generating large numbers of transposon mutants in clinically important strains of C. difficile is described and a method to study the function of every gene in the bacterium simultaneously is developed, identifying a set of 404 genes required for growth of the bacteria in the laboratory.

TL;DR: The results suggest that North American isolates of Pa have acquired virulence against man via the acquisition of a plasmid and specific virulence factors with similarity to those shown to play roles in pathogenicity against humans in other bacteria.

TL;DR: Analysis of the co-regulated subunits of a stage-specific enzyme is therefore a novel approach to uncover cryptic regulatory sequences controlling gene expression at the post-transcriptional level.