Matthew P. DeLisa

TL;DR: The author explains how the biotechnology and bioprocess engineering profession has changed in the modern era, and some examples of this change can be found in the chapters on Bioreactors for Suspension and Immobilized Cultures.

TL;DR: Several of nature's most notable multifunctional enzyme systems including the AROM complex and tryptophan synthase are discussed, each of which provides new fundamental insights into the structural organization of metabolic machinery within living cells.

TL;DR: It is demonstrated that in vivo only proteins that have attained the native conformation are exported by the Tat translocator, indicating that a folding quality-control mechanism is intrinsic to the export process.

TL;DR: This work investigated global changes in mRNA abundance elicited by the AI-2 signaling molecule through the use of a luxS mutant that was unable to synthesizeAI-2, and significant induction of ygeV, a putative sigma(54)-dependent transcriptional activator, and yhbH, a s Sigma(54) modulating protein, suggests sigma (54) may be involved in E. coli quorum sensing.

TL;DR: It is shown here that engineered Escherichia coli outer membrane vesicles (OMVs) are an easily purified vaccine-delivery system capable of greatly enhancing the immunogenicity of a low-immunogenicity protein antigen without added adjuvants.