Md. Zahid Kamal

TL;DR: Although the effect of all organic solvents on various properties on lipase is qualitatively similar, the study suggest that magnitudes of effects do not appear to follow bulk solvent properties like polarity and the solvent effects are apparently dictated by specific and local interactions of solvent molecule(s) with the protein.

TL;DR: The study suggest that better anchoring of the loops with the rest of the protein molecule through mutations particularly on the sites that perturb/disturb the exposed hydrophobic patches can simultaneously increase protein stability and aggregation resistance.

TL;DR: The study implies that widely accepted positive correlation between conformation flexibility and enzyme activity need not be stringent and draws attention to the possibility that high enzyme activity can still be accomplished in a rigid active site and stable protein structures.

TL;DR: Data suggest that unfolding of three domains of BSA follow the following order of susceptibility towards alkaline denaturation of bovine serum albumin: domain I>domain III>domain II.

TL;DR: Fluorescence-based dye-binding and agarose gel-based assays indicated that Lipid 1 binds to DNA more efficiently than Lipid 2 at charge ratios of >1:1 and the presence of amide linker apparently resulted in stable vesicle formation, higher melting temperature, and low compressibility, while retaining the membrane fluid properties suggesting that the intermolecular hydrogen bonds of Lipids 1 yielded stable lipoplexes of high transfection efficiency.