Michael J. Grey

TL;DR: Results suggest that linkage between ligand-induced dimerization and tyrosine kinase activation is much looser than was previously envisioned.

TL;DR: Conformational changes occurring on the microsecond-millisecond time scale in basic pancreatic trypsin inhibitor (BPTI) are investigated using nuclear magnetic resonance spectroscopy to characterize the kinetics and mechanism of chemical exchange line broadening of the backbone of Cys 14, Lys 15, Cys 38, and Arg 39 in BPTI.

TL;DR: NMR spin relaxation experiments are used to characterize the dynamics of the backbone of ubiquitin and chemical exchange processes affecting residues Ile 23, Asn 25, Thr 55, and Val 70 are identified using 1H‐15N multiple quantum relaxation experiments.

TL;DR: Rec recombinant EGFR constructs containing the extracellular, transmembrane, juxtamembrane and kinase domains are overexpressed and purified from human embryonic kidney 293 cell cultures and the oligomerization state, overall structure, and functional stability of the purified EGF-bound receptor are characterized in detergent micelles and phospholipid bilayers.

TL;DR: This chapter surveys the theoretical bases for TROSY in spin systems subject to chemical exchange linebroadening, the experimental methods that have been developed to quantitatively characterize chemical exchange in large proteins, and the emerging applications to triose phosphate isomerase, hemoglobin, and malate synthase G.