Showing papers by "Michel Aigle published in 2000"
TL;DR: A comparative genomics study of a homogeneous group of species classified as Hemiascomycetes, including Saccharomyces cerevisiae, allows to examine the conservation of chromosome maps, to identify the ‘yeast‐specific’ genes, and to review the distribution of gene families into functional classes.
226 citations
TL;DR: It is considered that distribution of S. bayanus var.
136 citations
TL;DR: Comparing the S. cerevisiae sequence with the other yeast species of this program indicates the existence of 50 novel genes in segments previously considered as ‘intergenic’ and suggests extensions for 26 of the previously annotated genes.
93 citations
TL;DR: A model is proposed for the molecular evolution of Hemiascomycetes based on the reiteration of events of duplication of chromosome segments, creating transient merodiploids that are subsequently resolved by single gene deletion events.
77 citations
TL;DR: Using genetic and flow cytometric analyses, it was shown that wine strain S6U is an allotetraploid of S. cerevisiae x S. bayanus and sherry strain S.BY 2592 was found to be an autotetra ploid heterozygous for homo-heterothallism.
66 citations
TL;DR: The overall degree of gene redundancy seems conserved across all species, best explained by a dynamic equilibrium resulting from numerous events of gene duplication and deletion rather than by a massive duplication event occurring in some lineages and not in others.
55 citations
TL;DR: The primary analysis of the sequences for the Hemiascomycete random sequence tag (RST) project was performed using a combination of classical methods for sequence comparison and contig assembly, and of specifically written scripts and computer visualization routines.
54 citations
TL;DR: It is shown that a majority of the ‘maverick’ genes have homologues among other yeast species and thus define a set of 1892 genes that, from sequence comparisons, appear ‘Ascomycetes‐specific’.
54 citations
TL;DR: Analysis of putative functions of the candidates confirm involvement of the Rvsp in endocytosis/vesicle traffic, but also opens possible new fields, such as nuclear functions.
Abstract: The Rvs161p and Rvs167p proteins of Saccharomyces cerevisiae, homologues of higher eukaryotes' amphiphysins, associate with actin and appear to be involved in several functions related to the actin cytoskeleton. In order to identify partners of the Rvsp proteins, yeast libraries constructed in two-hybrid vectors were screened using either Rvs167p or Rvs161p as a bait. The selected candidates, representing 34 ORFs, were then tested against both Rvsp proteins, as well as domains of Rvs167p or Rvs161p. Among the most significant ones, 24 ORFs were specific preys of Rvs167p only and two gave interactions with Rvs161p only. Interestingly, five ORFs were preys of both Rvs161p and Rvs167p (RVS167, LAS17, YNL094w, YMR192w and YPL249c). Analysis of putative functions of the candidates confirm involvement of the Rvsp in endocytosis/vesicle traffic, but also opens possible new fields, such as nuclear functions. Copyright © 2000 John Wiley & Sons, Ltd.
47 citations
TL;DR: The corresponding RSTs revealed that S. uvarum can exist without any S. cerevisiae DNA introgression, and cases in which synteny is lost suggest special mechanisms of genome evolution.
44 citations
TL;DR: Yeast biological diversity seems to result from limited species‐specific gene losses or duplications, and for a large part from rapid evolution of genes and regulatory factors dedicated to specific functions.