Molly Gasperini

TL;DR: Saturation genome editing is used to assay 96.5% of all possible single-nucleotide variants in 13 exons that encode functionally critical domains of BRCA1, and functional effects for nearly 4,000 SNVs are bimodally distributed and almost perfectly concordant with established assessments of pathogenicity.

TL;DR: A multiplex, expression quantitative trait locus (eQTL)-inspired framework for mapping enhancer-gene pairs by introducing random combinations of CRISPR/Cas9-mediated perturbations to each of many cells, followed by single-cell RNA sequencing (RNA-seq).

TL;DR: Gasserini et al. as mentioned in this paper reviewed emerging technologies for discovering, characterizing and validating human enhancers at scale and proposed a new framework for operationally defining enhancers that accommodates the heterogeneous and complementary results emerging from reporter assays, biochemical measurements and CRISPR screens.

TL;DR: This work optimized a published alternative, CROP-seq, in which the guide RNA also serves as the barcode, and here confirm that this strategy performs robustly and doubled the rate at which guides are assigned to cells to 94%.

TL;DR: The miR-199a-5p mature miRNA sequences are transcribed from stem loop precursor miRNAs that are found within the introns of the dynamin-2 and dynamin3 loci as discussed by the authors.