N. Puzenat

TL;DR: The glucose/insulin response element of the L-pyruvate kinase gene is a perfect palindrome located from nt -168 to -144 with respect to the cap site, and this element (L4) is partially homologous to MLTF binding sites.

TL;DR: A 183 base-pair fragment of the liver-specific promoter of the L-type puruvate kinase (L-PK) gene has been shown by transfection assay to be sufficient to confer a tissue-specific expression to a reporter gene.

TL;DR: The proximal 5'-flanking sequence of the L-PK gene appears to function in vitro as an efficient liver-specific promoter which requires the binding of the liver factor HNF1 and which is also stimulated by thebinding of another liver- specific factor, LF-A1.

TL;DR: A protein with a molecular mass of 19 kDa has been purified to homogeneity from 11-day-old chick embryos using a procedure involving chromatography on heparin - Sepharose, immunoaffinity resin and C4 reversed-phase and the first 17 amino acid residues of the N-terminal sequence show that it does not strictly correspond to any previously described protein.

TL;DR: In vivo and ex vivo experiments allowed us to established that thyroid hormones and glucocorticoids act on pyruvate kinase gene expression at the post-transcriptional level and to determine with accuracy the DNA elements responsible for response to diet and hormones.