Showing papers by "Nancy Kleckner published in 1984"
TL;DR: Several new variants of the tetracycline-resistance transposon Tn10 are described which are more useful than the wild-typetransposon for many types of genetic and physical analysis of bacteria.
621 citations
TL;DR: Three mutations of Escherichia coli K-12, designated texA, are described that enhance excision of Tn10 and of the structurally analogous transposon Tn5; for one texA allele, excision has become dependent on RecA function as well.
Abstract: Precise and nearly precise excision of transposon Tn10 occur by host-mediated processes unrelated to transposition. Both types of excision involve interactions between short (9 or 24 base-pair) direct repeat sequences at or near the termini of the transposon and are stimulated by the large (1,329-base-pair) inverted repeats that form the ends of Tn10. We describe here three mutations of Escherichia coli K-12, designated texA, that enhance excision of Tn10 and of the structurally analogous transposon Tn5. Genetic mapping and complementation analysis show that these mutations are unusual alleles of the recB and recC genes that alter but do not abolish RecBC function. As Tn10 excision normally does not depend on RecA or RecBC functions, texA mutations appear to provide another pathway for excision that depends on altered RecBC function; for one texA allele, excision has become dependent on RecA function as well. The available evidence suggests that texA mutations alter the stimulatory interaction between the inverted repeats of Tn10.
68 citations
TL;DR: These experiments show that transposase protein interacts preferentially with the genetically most active termini in a way that suppresses productive interaction with weaker termini present on the same substrate molecule.
63 citations
32 citations
TL;DR: It is concluded that at least 13 and no more than 27 base pairs of terminal IS10 sequence are absolutely required at each end for transposition of Tn10.
Abstract: We describe here point and deletion mutations that define which sequences at the termini of Tn10 are essential for transposition. We conclude that at least 13 and no more than 27 base pairs of terminal IS10 sequence are absolutely required at each end. These sequences correspond closely to the terminal inverted repeats of IS10. Sequences between base pairs 27 and 70 at each terminus and certain non-IS10 sequences can also influence transposition, but to a lesser degree. We also describe properties of many function-defective Tn10 transposition mutants and one exceptional Tn10 mutant.
30 citations
TL;DR: It is shown that a single Tn10 element, with its two closely spaced insertion sequence (IS10) elements, frequently gives rise to complex rearrangements that can be accounted for as the sum of two "classical" IS10 events.
20 citations