Naomi C. Franklin

TL;DR: Two multiple-copy, ColE1-type, plasmid cloning vehicles, pHUB2 and pHUB4, have been constructed that carry four different single restriction sites down-stream from the phage lambda promoter pL, and pL dependent expression of the inserted gene in Escherichia coli was determined by measuring the enzymatic activity of the trpA gene product.

TL;DR: It is concluded that transcription initiated at p L by E. coli RNA polymerase is so modified by interaction with N protein that even distant nonsense codons are no longer read as a cause of polarity.

TL;DR: Nucleotide sequence has been determined for the restriction fragments and cloned DNA from the pL-N-tL1 region of bacteriophage lambda, finding the protein coded is 133 or, more probably, 107 amino acids long, rich in lysine, arginine and proline.