N
Neil R. Gilkes
Researcher at University of British Columbia
Publications - 111
Citations - 11881
Neil R. Gilkes is an academic researcher from University of British Columbia. The author has contributed to research in topics: Cellulase & Cellulose. The author has an hindex of 58, co-authored 111 publications receiving 11475 citations.
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Journal ArticleDOI
Enhancement of the Endo-β-1,4-glucanase Activity of an Exocellobiohydrolase by Deletion of a Surface Loop
Andreas Meinke,H. G. Damude,P. Tomme,Emily Kwan,Douglas G. Kilburn,Robert C. Miller,R. Antony J. Warren,Neil R. Gilkes +7 more
TL;DR: The mutation enhanced the endoglucanase activity of the enzyme on soluble O-(carboxymethyl)cellulose and altered its activities on 2′,4′-dinitrophenyl-β-D-cellobioside, insoluble cellulose, and cellotetraose and the hypothesis that hydrolysis by Trichoderma reesei cellobiohydrolase II is restricted to the ends of cellulose polymers was examined.
Journal ArticleDOI
Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities.
TL;DR: Analysis of carboxymethyl cellulose hydrolysis suggests that CenC is semiprocessive enzyme with both endo- and exoglucanase activities.
Journal ArticleDOI
Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose.
TL;DR: CenA′‐′PhoA fusion polypeptides which contain the entire cellulose‐binding domain of CenA bind to cellulose, allowing their purification from periplasmic extracts in a single, facile step and has implications for purification or immobilisation of chimeric proteins on a cheap cellulose matrix.
Journal ArticleDOI
Direct 1H N.M.R. determination of the stereochemical course of hydrolyses catalysed by glucanase components of the cellulase complex
Stephen G. Withers,Dennise Dombroski,Leise A. Berven,Douglas G. Kilburn,Robert C. Miller,R. Anthony J. Warren,Neil R. Gilkes +6 more
TL;DR: 1H nmr approach has several advantages over other techniques in that it is applicable to a wide variety of glycosidases and substrates and it is non-destructive, allowing recovery of the enzyme.
Journal ArticleDOI
The purification and characterization of Dictyostelium discoideum plasma membranes.
Neil R. Gilkes,Gerald Weeks +1 more
TL;DR: A new procedure for the purification of plasma membranes of Dictyostelium discoideum is described, which is compared to previously published procedures which have been found to be unsuitable for the authors' purposes.