N
Nicholas M. Gough
Researcher at Walter and Eliza Hall Institute of Medical Research
Publications - 72
Citations - 8380
Nicholas M. Gough is an academic researcher from Walter and Eliza Hall Institute of Medical Research. The author has contributed to research in topics: Leukemia inhibitory factor & Gene. The author has an hindex of 41, co-authored 72 publications receiving 8270 citations. Previous affiliations of Nicholas M. Gough include Ludwig Institute for Cancer Research.
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Journal ArticleDOI
Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells
Rachel Williams,D J Hilton,S Pease,Tracy A. Willson,Colin L. Stewart,David P. Gearing,Erwin F. Wagner,D. Metcalf,Nicos A. Nicola,Nicholas M. Gough +9 more
TL;DR: In this paper, a recombinant myeloid leukaemia inhibitory factor (LIF) was used to replace DIA in the maintenance of totipotent ES cell lines that retain the potential to form chimaeric mice.
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Expression cloning of a receptor for human granulocyte-macrophage colony-stimulating factor.
TL;DR: Two cDNA clones encoding a receptor for human granulocyte‐macrophage colony‐stimulating factor were isolated by expression screening of a library made from human placental mRNA and cross‐linking experiments revealed a similar size for the glycosylated receptors in transfected COS and haemopoietic cells.
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Molecular cloning and expression of cDNA encoding a murine myeloid leukaemia inhibitory factor (LIF).
David P. Gearing,Nicholas M. Gough,Julie King,D J Hilton,Nicos A. Nicola,Richard J. Simpson,Edouard C. Nice,Anne Kelso,D. Metcalf +8 more
TL;DR: Cloning, sequencing and expressing LIF has resolved several discrepancies in the literature concerning the identity of factors capable of inducing differentiation of murine myeloid leukaemic cells in vitro.
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Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor.
Nicholas M. Gough,Jill Gough,Donald Metcalf,Anne Kelso,Dianne Grail,Nicos A. Nicola,Antony W. Burgess,Ashley R. Dunn +7 more
TL;DR: cDNA clones specifying the murine granulocyte–macrophage colony stimulating factor have been isolated and it bears no structural similarity to the functionally related factor, interleukin-3, described recently.
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Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells
TL;DR: An extremely simple procedure for preparing cytoplasmic RNA from small numbers of cells is described and RNA is ready for Northern gel analysis in less than 30 min.